EDF1 cdna clone

EDF1

Cat. Num. AAA200567

• Synonyms: EDF1; EDF-1; MBF1; EDF1 cdna clone

• Form/Format: Lyophilized
• Sequence: Nucleotide Sequence: ATGGCCGAGA GCGACTGGGA CACGGTGACG GTGCTGCGCA AGAAGGGCCC TACGGCCGCC CAGGCCAAAT CCAAGCAGGC TATCTTAGCG GCACAGAGAC GAGGAGAAGA TGTGGAGACT TCCAAGAAAT GGGCTGCTGG CCAGAACAAA CAACATTCTA TTACCAAGAA CACGGCCAAG CTGGACCGGG AGACAGAGGA GCTGCACCAT GACAGGGTGA CCCTGGAGGT GGGCAAGGTG ATCCAGCAAG GTCGGCAGAG CAAGGGGCTT ACGCAGAAGG ACCTGGCCAC GAAAATCAAT GAGAAGCCAC AGGTGATCGC GGACTATGAG AGCGGACGGG CCATACCCAA TAACCAGGTG CTTGGCAAAA TCGAGCGGGC CATTGGCCTC AAGCTCCGGG GAAAGGACAT TGGAAAGCCC ATCGAGAAGG GGCCTAGGGC GAAATGA; ; Translation Sequence: MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAK
• Sequence Length: 148

• Species: Human
• Chromosome Location: 9q34.3
• OMIM Reference Number: 605107
• cDNA Size: 447bp
• Vector Description: This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.
• Vector: (puc19-derived cloning vector)
• Preparation Before Usage: 1. Centrifuge at 7000rpm for 1 minute. 2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA. Each tube contains approximately 10ug of lyophilized plasmid.
• Preparation and Storage: Store the plasmid at -20 degree C.

Related Product Information for EDF1 cdna clone

Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas.

Product Categories/Family for EDF1 cdna clone

Epigenomics (Transcription & Translation); Metabolism; Differentiation & Regeneration

NCBI and Uniprot Product Information

• NCBI GI #: 4503453
• NCBI Accession #: NP_003783.1
• NCBI GenBank Nucleotide #: NM_003792.3
• UniProt Accession #: O60869
• NCBI Official Full Name: endothelial differentiation-related factor 1 isoform alpha
• UniProt Protein Name: Endothelial differentiation-related factor 1
• Protein Family: Endothelial differentiation-related factor
• UniProt Gene Name: EDF1
• UniProt Synonym Gene Names: EDF-1; MBF1
• UniProt Entry Name: EDF1_HUMAN

Uniprot Description

EDF1: Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; DNA-binding; Nuclear receptor co-regulator; RNA-binding

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: transcription factor TFIID complex; cytoplasm; nucleolus; intracellular; nucleus

Molecular Function: calmodulin binding; methyltransferase activity; protein binding; histone acetyltransferase activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity

Biological Process: endothelial cell differentiation; regulation of lipid metabolic process; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of DNA binding