EARS2 cdna clone

EARS2 cDNA Clone

Cat. Num. AAA1271272

• Gene Names: EARS2; MSE1; gluRS; COXPD12
• Synonyms: EARS2; EARS2 cDNA Clone; EARS2 cdna clone

• Sequence: atggcggcgctcctgaggagactgctgcagcgcgagaggccttcggcggcctctggccgccccgtaggacggcgcgaggccaacctgggcactgatgccggggttgcggtgcgagtgcggttcgctcccagccccacaggcttcttgcacctgggtggcctccgcactgccttgtacaactacatctttgctaagaagtaccaggggagcttcatcctgaggctagaggacacagatcagactcgcgttgtgcctggggcagcagagaatattgaggacatgctggagtgggcaggcatcccgcctgatgagagcccccgccggggcggtcctgctgggccctaccagcaatctcagcggttggagctgtatgcccaggccacagaagcgctgctgaagaccggagctgcttacccctgtttctgctcaccccagcggctggagctcctgaagaaggaggccttgcggaaccaccagacgccccggtatgacaatcggtgcaggaacatgagccaggagcaggtggcccagaagctggccaaggaccccaagcctgcgatccgcttccgcctggagcaggtggtgccagccttccaggacctggtgtatggctggaataggcatgaagtggccagcgtggagggagacccagtcatcatgaagagcgacggcttccccacataccacctggcctgcgtggtggacgaccaccacatgggcatcagccacgtgctgcgaggctctgagtggctcgtctccactgccaagcacctgctcctctaccaggccctgggctggcagccaccccacttcgcccacctgcccctgctcctcaacagggatggcagcaagctctccaagaggcaaggggacgttttcctggagcactttgctgctgatggcttcctgcccgattccttgttggacatcatcaccaactgtggctcaggttttgcagagaaccaaatgggcaggaccctgccggagctgatcacacagttcaacctgacacaggtcacctgtcactcagccctgctggacctggagaagctcccagaattcaacagactgcacctccagcggctggtgagcaatgagagccagaggcgccagctggtggggaagctgcaggtccttgtggaggaggcctttggttgccagctgcaaaacagggatgtcctcaacccagtctacgtggagaggatcctcctgctgagacagggtcacatttgccgcctgcaggacttggtgtccccagtatactcttacctgtggactcgccctgcagtaggtcgagcacagctggacgccatctcggagaaggtggatgtgattgccaagcgtgtgctggggcttctagaaagatctggtatgagcttaactcaggatatgctgaatggagaactgaagaagctatcagaaggtttggaaggcaccaagtacagtaatgtgatgaaactccttcggatggccctcagtggacagcaggtgaggcagggacacgggttggattgttccctggagcccctcattgatcctttgaacttacatttcctggcaggcactgagctcaatattgagtatacaaaggtgaatgaaacatga
• Sequence Length: 1605

• Vector: pENTR223.1 or pUC
• Clone Sequence Report: Provided with product shipment

NCBI and Uniprot Product Information

• NCBI GI #: 25058988
• NCBI GeneID: 124454
• Molecular Weight: 59,989 Da
• NCBI Official Full Name: Homo sapiens glutamyl-tRNA synthetase 2, mitochondrial (putative), mRNA
• NCBI Official Synonym Full Names: glutamyl-tRNA synthetase 2, mitochondrial
• NCBI Official Symbol: EARS2
• NCBI Official Synonym Symbols: MSE1; gluRS; COXPD12
• NCBI Protein Information: probable glutamate--tRNA ligase, mitochondrial
• UniProt Protein Name: Probable glutamate--tRNA ligase, mitochondrial
• Protein Family: Probable glutamate--tRNA ligase
• UniProt Gene Name: EARS2
• UniProt Synonym Gene Names: KIAA1970; GluRS
• UniProt Entry Name: SYEM_HUMAN

NCBI Description

This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

EARS2: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Translation; Mitochondrial; EC 6.1.1.17; Ligase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion

Molecular Function: glutamate-tRNA ligase activity; glutamate-tRNA(Gln) ligase activity; RNA binding

Biological Process: glutamyl-tRNA aminoacylation

Disease: Combined Oxidative Phosphorylation Deficiency 12