BUB1 cdna clone
BUB1 cDNA Clone
Gene Names
BUB1; BUB1A; BUB1L; hBUB1
Synonyms
BUB1; BUB1 cDNA Clone; BUB1 cdna clone
Sequence
atggacaccccggaaaatgtccttcagatgcttgaagcccacatgcagagctacaagggcaatgaccctcttggtgaatgggaaagatacatacagtgggtagaagagaattttcctgagaataaagaatacttgataactttactagaacatttaatgaaggaatttttagataagaagaaataccacaatgacccaagattcatcagttattgtttaaaatttgctgagtacaacagtgacctccatcaattttttgagtttctgtacaaccatgggattggaaccctgtcatcccctctgtacattgcctgggcggggcatctggaagcccaaggagagctgcagcatgccagtgctgtccttcagagaggaattcaaaaccaggctgaacccagagagttcctgcaacaacaatacaggttatttcagacacgcctcactgaaacccatttgccagctcaagctagaacctcagaacctctgcataatgttcaggttttaaatcaaatgataacatcaaaatcaaatccaggaaataacatggcctgcatttctaagaatcagggttcagagctttctggagtgatatcttcagcttgtgataaagagtcaaatatggaacgaagagtgatcacgatttctaaatcagaatattctgtgcactcatctttggcatccaaagttgatgttgagcaggttgttatgtattgcaaggagaagcttattcgtggggaatcagaattttcctttgaagaattgagagcccagaaatacaatcaacggagaaagcatgagcaatgggtaaatgaagacagacattatatgaaaaggaaagaagcaaatgcttttgaagaacagctattaaaacagaaaatggatgaacttcataagaagttgcatcaggtggtggagacatcccatgaggatctgcccgcttcccaggaaaggtccgaggttaatccagcacgtatggggccaagtgtaggctcccagcaggaactgagagcgccatgtcttccagtaacctatcagcagacaccagtgaacatggaaaagaacccaagagaggcacctcctgttgttcctcctttggcaaatgctatttctgcagctttggtgtccccagccaccagccagagcattgctcctcctgttcctttgaaagcccagacagtaacagactccatgtttgcagtggccagcaaagatgctggatgtgtgaataagagtactcatgaattcaagccacagagtggagcagagatcaaagaagggtgtgaaacacataaggttgccaacacaagttcttttcacacaactccaaacacatcactgggaatggttcaggcaacgccatccaaagtgcagccatcacccaccgtgcacacaaaagaagcattaggtttcatcatgaatatgtttcaggctcctacacttcctgatatttctgatgacaaagatgaatggcaatctctagatcaaaatgaagatgcatttgaagcccagtttcaaaaaaatgtaaggtcatctggggcttggggagtcaataagatcatctcttctttgtcatctgcttttcatgtgtttgaagatggaaacaaagaaaattatggattaccacagcctaaaaataaacccacaggagccaggacctttggagaacgctctgtcagcagacttccttcaaaaccaaaggaggaagtgcctcatgctgaagagtttttggatgactcaactgtatggggtattcgctgcaacaaaaccctggcacccagtcctaagagcccaggagacttcacatctgctgcacaacttgcgtctacaccattccacaagcttccagtggagtcagtgcacattttagaagataaagaaaatgtggtagcaaaacagtgtacccaggcgactttggattcttgtgaggaaaacatggtggtgccttcaagggatggaaaattcagtccaattcaagagaaaagcccaaaacaggccttgtcgtctcacatgtattcagcatccttacttcgtctgagccagcctgctgcaggtggggtacttacctgtgaggcagagttgggcgttgaggcttgcagactcacagacactgacgctgccattgcagaagatccaccagatgctattgctgggctccaagcagaatggatgcagatgagttcacttgggactgttgatgctccaaacttcattgttgggaacccatgggatgataagctgattttcaaacttttatctgggctttctaaaccagtgagttcctatccaaatacttttgaatggcaatgtaaacttccagccatcaagcccaagactgaatttcaattgggttctaagctggtctatgtccatcaccttcttggagaaggagcctttgcccaggtgtacgaagctacccagggagatctgaatgatgctaaaaataaacagaaatttgttttaaaggtccaaaagcctgccaacccctgggaattctacattgggacccagttgatggaaagactaaagccatctatgcagcacatgtttatgaagttctattctgcccacttattccagaatggcagtgtattagtaggagagctctacagctatggaacattattaaatgccattaacctctataaaaatacccctgaaaaagtgatgcctcaaggtcttgtcatctcttttgctatgagaatgctttacatgattgagcaagtgcatgactgtgaaatcattcatggagacattaaaccagacaatttcatacttggaaacggatttttggaacaggatgatgaagatgatttatctgctggcttggcactgattgacctgggtcagagtatagatatgaaactttttccaaaaggaactatattcacagcaaagtgtgaaacatctggttttcagtgtgttgagatgctcagcaacaaaccatggaactaccagatcgattactttggggttgctgcaacagtatattgcatgctctttggcacttacatgaaagtgaaaaatgaaggaggagagtgtaagcctgaaggtctttttagaaggcttcctcatttggatatgtggaatgaattttttcatgttatgttgaatattccagattgtcatcatcttccatctttggatttgttaaggcaaaagctgaagaaagtatttcaacaacactatactaacaagattagggccctacgtaataggctaattgtactgctcttagaatgtaagcgttcacgaaaataa
Sequence Length
3258
Vector
pUC
Clone Sequence Report
Provided with product shipment
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
120,002 Da
NCBI Official Full Name
Homo sapiens budding uninhibited by benzimidazoles 1 homolog (yeast), mRNA
NCBI Official Synonym Full Names
BUB1 mitotic checkpoint serine/threonine kinase
NCBI Official Symbol
BUB1
NCBI Official Synonym Symbols
BUB1A; BUB1L; hBUB1
NCBI Protein Information
mitotic checkpoint serine/threonine-protein kinase BUB1
UniProt Protein Name
Mitotic checkpoint serine/threonine-protein kinase BUB1
Protein Family
UniProt Gene Name
BUB1
UniProt Synonym Gene Names
BUB1L; hBUB1
UniProt Entry Name
BUB1_HUMAN
NCBI Description
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.
Protein type: Kinase, protein; Protein kinase, Other; Cell cycle regulation; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; BUB family
Chromosomal Location of Human Ortholog: 2q14
Cellular Component: cytoplasm; cytosol; kinetochore; membrane; nucleoplasm
Molecular Function: protein binding; protein kinase activity
Biological Process: mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; regulation of sister chromatid cohesion; sister chromatid cohesion
Research Articles on BUB1
Similar Products
Product Notes
The BUB1 bub1 (Catalog #AAA1267055) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: atggacaccc cggaaaatgt ccttcagatg cttgaagccc acatgcagag ctacaagggc aatgaccctc ttggtgaatg ggaaagatac atacagtggg tagaagagaa ttttcctgag aataaagaat acttgataac tttactagaa catttaatga aggaattttt agataagaag aaataccaca atgacccaag attcatcagt tattgtttaa aatttgctga gtacaacagt gacctccatc aattttttga gtttctgtac aaccatggga ttggaaccct gtcatcccct ctgtacattg cctgggcggg gcatctggaa gcccaaggag agctgcagca tgccagtgct gtccttcaga gaggaattca aaaccaggct gaacccagag agttcctgca acaacaatac aggttatttc agacacgcct cactgaaacc catttgccag ctcaagctag aacctcagaa cctctgcata atgttcaggt tttaaatcaa atgataacat caaaatcaaa tccaggaaat aacatggcct gcatttctaa gaatcagggt tcagagcttt ctggagtgat atcttcagct tgtgataaag agtcaaatat ggaacgaaga gtgatcacga tttctaaatc agaatattct gtgcactcat ctttggcatc caaagttgat gttgagcagg ttgttatgta ttgcaaggag aagcttattc gtggggaatc agaattttcc tttgaagaat tgagagccca gaaatacaat caacggagaa agcatgagca atgggtaaat gaagacagac attatatgaa aaggaaagaa gcaaatgctt ttgaagaaca gctattaaaa cagaaaatgg atgaacttca taagaagttg catcaggtgg tggagacatc ccatgaggat ctgcccgctt cccaggaaag gtccgaggtt aatccagcac gtatggggcc aagtgtaggc tcccagcagg aactgagagc gccatgtctt ccagtaacct atcagcagac accagtgaac atggaaaaga acccaagaga ggcacctcct gttgttcctc ctttggcaaa tgctatttct gcagctttgg tgtccccagc caccagccag agcattgctc ctcctgttcc tttgaaagcc cagacagtaa cagactccat gtttgcagtg gccagcaaag atgctggatg tgtgaataag agtactcatg aattcaagcc acagagtgga gcagagatca aagaagggtg tgaaacacat aaggttgcca acacaagttc ttttcacaca actccaaaca catcactggg aatggttcag gcaacgccat ccaaagtgca gccatcaccc accgtgcaca caaaagaagc attaggtttc atcatgaata tgtttcaggc tcctacactt cctgatattt ctgatgacaa agatgaatgg caatctctag atcaaaatga agatgcattt gaagcccagt ttcaaaaaaa tgtaaggtca tctggggctt ggggagtcaa taagatcatc tcttctttgt catctgcttt tcatgtgttt gaagatggaa acaaagaaaa ttatggatta ccacagccta aaaataaacc cacaggagcc aggacctttg gagaacgctc tgtcagcaga cttccttcaa aaccaaagga ggaagtgcct catgctgaag agtttttgga tgactcaact gtatggggta ttcgctgcaa caaaaccctg gcacccagtc ctaagagccc aggagacttc acatctgctg cacaacttgc gtctacacca ttccacaagc ttccagtgga gtcagtgcac attttagaag ataaagaaaa tgtggtagca aaacagtgta cccaggcgac tttggattct tgtgaggaaa acatggtggt gccttcaagg gatggaaaat tcagtccaat tcaagagaaa agcccaaaac aggccttgtc gtctcacatg tattcagcat ccttacttcg tctgagccag cctgctgcag gtggggtact tacctgtgag gcagagttgg gcgttgaggc ttgcagactc acagacactg acgctgccat tgcagaagat ccaccagatg ctattgctgg gctccaagca gaatggatgc agatgagttc acttgggact gttgatgctc caaacttcat tgttgggaac ccatgggatg ataagctgat tttcaaactt ttatctgggc tttctaaacc agtgagttcc tatccaaata cttttgaatg gcaatgtaaa cttccagcca tcaagcccaa gactgaattt caattgggtt ctaagctggt ctatgtccat caccttcttg gagaaggagc ctttgcccag gtgtacgaag ctacccaggg agatctgaat gatgctaaaa ataaacagaa atttgtttta aaggtccaaa agcctgccaa cccctgggaa ttctacattg ggacccagtt gatggaaaga ctaaagccat ctatgcagca catgtttatg aagttctatt ctgcccactt attccagaat ggcagtgtat tagtaggaga gctctacagc tatggaacat tattaaatgc cattaacctc tataaaaata cccctgaaaa agtgatgcct caaggtcttg tcatctcttt tgctatgaga atgctttaca tgattgagca agtgcatgac tgtgaaatca ttcatggaga cattaaacca gacaatttca tacttggaaa cggatttttg gaacaggatg atgaagatga tttatctgct ggcttggcac tgattgacct gggtcagagt atagatatga aactttttcc aaaaggaact atattcacag caaagtgtga aacatctggt tttcagtgtg ttgagatgct cagcaacaaa ccatggaact accagatcga ttactttggg gttgctgcaa cagtatattg catgctcttt ggcacttaca tgaaagtgaa aaatgaagga ggagagtgta agcctgaagg tctttttaga aggcttcctc atttggatat gtggaatgaa ttttttcatg ttatgttgaa tattccagat tgtcatcatc ttccatcttt ggatttgtta aggcaaaagc tgaagaaagt atttcaacaa cactatacta acaagattag ggccctacgt aataggctaa ttgtactgct cttagaatgt aagcgttcac gaaaataa. It is sometimes possible for the material contained within the vial of "BUB1, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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