SDS-PAGE
CDKL1 recombinant protein
CDKL1 recombinant protein
Gene Names
CDKL1; P42; KKIALRE
Applications
Western Blot
Synonyms
CDKL1; CDKL1 recombinant protein; KKIALRE; P42; CDKL1 Protein
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
1619
Applicable Applications for CDKL1 recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human CDKL1 was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for CDKL1 recombinant protein
Recombinant full-length human CDKL1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: CDKL1 or cyclin-dependent kinase-like 1 is a member of a large family of CDC2-related serine/threonine protein kinases. CDKL1, which is also known as p42 KKIALRE, accumulates primarily in the nucleus and is known to regulate important transitions in the eukaryotic cell cycle (1). CDKL1 and the related CDC2 protein kinase have a similar pattern of mRNA expression except in the reproductive organs. CDKL1 is expressed in several human tissues, with the highest expression in kidney and ovary (2). CDKL1 is overexpressed in breast cancer patients and has a positive detection efficiency of 77%. Inhibiting CDKL1 function with shRNA in MCF-7 cells shows increased accumulation of the cells at the G2/M phase and increased sensitivity to cell cycle chemotherapeutic drugs.
Scientific Background: CDKL1 or cyclin-dependent kinase-like 1 is a member of a large family of CDC2-related serine/threonine protein kinases. CDKL1, which is also known as p42 KKIALRE, accumulates primarily in the nucleus and is known to regulate important transitions in the eukaryotic cell cycle (1). CDKL1 and the related CDC2 protein kinase have a similar pattern of mRNA expression except in the reproductive organs. CDKL1 is expressed in several human tissues, with the highest expression in kidney and ovary (2). CDKL1 is overexpressed in breast cancer patients and has a positive detection efficiency of 77%. Inhibiting CDKL1 function with shRNA in MCF-7 cells shows increased accumulation of the cells at the G2/M phase and increased sensitivity to cell cycle chemotherapeutic drugs.
Product Categories/Family for CDKL1 recombinant protein
References
1. Meyerson, M.et.al: A family of human cdc2-related protein kinases. EMBO J. 11: 2909-2917, 1992.
2. Taglienti, C. A. et.al: Molecular cloning of the epidermal growth factor-stimulated protein kinase p56 KKIAMRE. Oncogene 13: 2563-2574, 1996.
2. Taglienti, C. A. et.al: Molecular cloning of the epidermal growth factor-stimulated protein kinase p56 KKIAMRE. Oncogene 13: 2563-2574, 1996.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~60 kDa
NCBI Official Full Name
Homo sapiens cyclin-dependent kinase-like 1 (CDC2-related kinase) (CDKL1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
cyclin-dependent kinase-like 1 (CDC2-related kinase)
NCBI Official Symbol
CDKL1
NCBI Official Synonym Symbols
P42; KKIALRE
NCBI Protein Information
cyclin-dependent kinase-like 1; CDC2-related kinase 1; protein kinase p42 KKIALRE; serine/threonine protein kinase KKIALRE; serine/threonine-protein kinase KKIALRE
UniProt Protein Name
Cyclin-dependent kinase-like 1
Protein Family
UniProt Gene Name
CDKL1
UniProt Entry Name
CDKL1_HUMAN
NCBI Description
This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
Uniprot Description
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Subcellular location: Cytoplasm. Nucleus Ref.6.
Tissue specificity: Highly expressed in kidney, and to a lower extent in ovary. Ref.6
Domain: The [NKR]KIAxRE motif seems to be a cyclin-binding region.
Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.
Sequence caution: The sequence AAI04978.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence AAS00095.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAA47002.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Research Articles on CDKL1
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Product Notes
The CDKL1 cdkl1 (Catalog #AAA515414) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human CDKL1 was expressed by baculovirus in Sf9 insect cells. AAA Biotech's CDKL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the CDKL1 cdkl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDKL1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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