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Threonylcarbamoyladenosine tRNA methylthiotransferase (Cdkal1) Recombinant Protein | Cdkal1 recombinant protein

Recombinant Mouse Threonylcarbamoyladenosine tRNA methylthiotransferase (Cdkal1)

Gene Names
Cdkal1; 1190005B03Rik; 6620401C13Rik
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Threonylcarbamoyladenosine tRNA methylthiotransferase (Cdkal1); Recombinant Mouse Threonylcarbamoyladenosine tRNA methylthiotransferase (Cdkal1); Cdkal1 recombinant protein
Ordering
For Research Use Only!
Host
Cell Free Expression
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
1-578. Full Length
Sequence
MPSASCDVLLDDIEDIISQEDSKPQDRQFSRKHVFPKVRRRNTQKYLQEEPRPPSDSTIPGIQKIWIRTWGCSHNNSDGEYMAGQLAAYGYKITENASDADLWLLNSCTVKNPAEDHFRNSIKKAQEENKKVVLAGCVPQAQPRQDYLKGLSIIGVQQIDRVVEVVEETIKGHSVRLLGQKKDNGKRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGNLASYPIDELVERAKQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWKLVEVIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHIPVQSASDSVLMDMKREYCVADFKRVVDFLKEKVPGITIATDIICGFPGETDQDFQETVKLVEEYKFPSLFINQFYPRPGTPAAKAEQVPAHVKKQRTKDLSRVFHSYNPYDHKIGERQQVLVTEESFDSKFYVAHNRFYEQVLVPKNPAFMGKMVEVDIYESGKHFLKGQPVSETRVYTPSISKPLAKGEVSGLTKEFRNRLGNHPNGTSDTCPATQHGSAYSRMVLQMSQYDCALKVATGLALLALLLHFWPDSLLTM
Application Notes
This is a recombinant transmembrane protein expressed in a cell-free expression system.
Species
Mouse
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Product Categories/Family for Cdkal1 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,558 Da
NCBI Official Full Name
threonylcarbamoyladenosine tRNA methylthiotransferase isoform 2
NCBI Official Synonym Full Names
CDK5 regulatory subunit associated protein 1-like 1
NCBI Official Symbol
Cdkal1
NCBI Official Synonym Symbols
1190005B03Rik; 6620401C13Rik
NCBI Protein Information
threonylcarbamoyladenosine tRNA methylthiotransferase
UniProt Protein Name
Threonylcarbamoyladenosine tRNA methylthiotransferase
UniProt Gene Name
Cdkal1
UniProt Entry Name
CDKAL_MOUSE

Uniprot Description

CDKAL1: Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. Belongs to the methylthiotransferase family. CDKAL1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 2.8.4.5; Enzyme, misc.

Cellular Component: endoplasmic reticulum; integral to membrane; membrane; rough endoplasmic reticulum

Molecular Function: 4 iron, 4 sulfur cluster binding; catalytic activity; iron-sulfur cluster binding; metal ion binding; transferase activity

Biological Process: biopolymer modification; RNA modification; tRNA processing

Research Articles on Cdkal1

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Product Notes

The Cdkal1 cdkal1 (Catalog #AAA7011216) is a Recombinant Protein produced from Cell Free Expression and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-578. Full Length. This is a recombinant transmembrane protein expressed in a cell-free expression system. Researchers should empirically determine the suitability of the Cdkal1 cdkal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPSASCDVLL DDIEDIISQE DSKPQDRQFS RKHVFPKVRR RNTQKYLQEE PRPPSDSTIP GIQKIWIRTW GCSHNNSDGE YMAGQLAAYG YKITENASDA DLWLLNSCTV KNPAEDHFRN SIKKAQEENK KVVLAGCVPQ AQPRQDYLKG LSIIGVQQID RVVEVVEETI KGHSVRLLGQ KKDNGKRLGG ARLDLPKIRK NPLIEIISIN TGCLNACTYC KTKHARGNLA SYPIDELVER AKQSFQEGVC EIWLTSEDTG AYGRDIGTDL PTLLWKLVEV IPEGAMLRLG MTNPPYILEH LEEMAKILNH PRVYAFLHIP VQSASDSVLM DMKREYCVAD FKRVVDFLKE KVPGITIATD IICGFPGETD QDFQETVKLV EEYKFPSLFI NQFYPRPGTP AAKAEQVPAH VKKQRTKDLS RVFHSYNPYD HKIGERQQVL VTEESFDSKF YVAHNRFYEQ VLVPKNPAFM GKMVEVDIYE SGKHFLKGQP VSETRVYTPS ISKPLAKGEV SGLTKEFRNR LGNHPNGTSD TCPATQHGSA YSRMVLQMSQ YDCALKVATG LALLALLLHF WPDSLLTM . It is sometimes possible for the material contained within the vial of "Threonylcarbamoyladenosine tRNA methylthiotransferase (Cdkal1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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