CDK2AP2 recombinant protein

CDK2AP2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121187

• Gene Names: CDK2AP2; p14; DOC-1R
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CDK2AP2; CDK2AP2 Protein; Human; Recombinant (His Tag); Human CDK2AP2 Protein (His Tag); DOC-1R Protein; p14 Protein; cyclin-dependent kinase 2 associated protein 2; CDK2AP2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 15% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr126

• Species: Human
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CDK2AP2 (O75956)(Met 1-Thr 126) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDK2AP2 recombinant protein

Background: CDK2AP2 belongs to the CDK2AP family. Members of this family of proteins are cell-growth suppressors, associating with and influencing the biological activities of important cell cycle regulators in the S phase including monomeric non-phosphorylated cyclin-dependent kinase 2 (CDK2) and DNA polymerase alpha/primase. CDK2AP2 contains 5 distinct gt-ag introns. Transcription produces 7 different mRNAs, 6 alternatively spliced variants and 1 unspliced form. There are 2 non overlapping alternative last exons and 4 validated alternative polyadenylation sites. The mRNAs appear to differ splicing versus retention of 3 introns. CDK2AP2 plays a role in regulating self-renewal of mouse embryonic stem cells (mESC) under permissive conditions, and cell survival during differentiation of the mESC into terminally differentiated cell types.

References

Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-68.2 Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.3 Buajeeb W, et al. (2004) Interaction of the CDK2-associated protein-1, p12(DOC-1/CDK2AP1), with its homolog, p14(DOC-1R). Biochem Biophys Res Commun. 315(4):998-1003

NCBI and Uniprot Product Information

• NCBI GI #: 5031669
• NCBI GeneID: 10263
• NCBI Accession #: NP_005842.1
• NCBI GenBank Nucleotide #: NM_005851.4
• UniProt Accession #: O75956
• Molecular Weight: 13,101 Da
• NCBI Official Full Name: cyclin-dependent kinase 2-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2 associated protein 2
• NCBI Official Symbol: CDK2AP2
• NCBI Official Synonym Symbols: p14; DOC-1R
• NCBI Protein Information: cyclin-dependent kinase 2-associated protein 2; DOC-1-related protein; CDK2-associated protein 2; tumor suppressor deleted in oral cancer related 1
• UniProt Protein Name: Cyclin-dependent kinase 2-associated protein 2
• Protein Family: Cyclin-dependent kinase 2-associated protein
• UniProt Gene Name: CDK2AP2
• UniProt Synonym Gene Names: DOC1R; CDK2-associated protein 2; DOC-1R
• UniProt Entry Name: CDKA2_HUMAN

NCBI Description

This gene encodes a protein that interacts with cyclin-dependent kinase 2 associated protein 1. Pseudogenes associated with this gene are located on chromosomes 7 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CDK2AP2: Belongs to the CDK2AP family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 11q13