SDS-PAGE
PFTK1 (CDK14) recombinant protein
PFTK1 (CDK14) recombinant protein
SDS-PAGE
SDS-PAGE
Scientific Background: PFTK1, also known as PFTAIRE protein kinase 1, is a member of the CDC2 -related protein kinase family which is expressed primarily in the postnatal and adult nervous system (1). PFTK1 is highly expressed in brain, pancreas, kidney, heart, testis, and ovary. PFTK1 interacts with 14-3-3-beta, 14-3-3-epsilon, 14-3-3-eta and 14-3-3-tau. Using PFTK1 mutant constructs and in vitro and in vivo binding studies, it was shown that PFTK1 amino acid residue ser119 is required for its interaction with all four 14-3-3 isoforms. (2). Significant upregulation of PFTK1 expression is observed in oesophageal squamous cell carcinoma (ESCC). PFTK1 is not only useful as a prognostic marker in ESCC, but also as a predictor of the response to chemotherapy.
2. Gao, Y. et.al: A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins. Cell Res. 16: 539-547, 2006.
NCBI and Uniprot Product Information
NCBI Description
PFTK1 is a member of the CDC2 (MIM 116940)-related protein kinase family (Yang and Chen, 2001 [PubMed 11313143]).[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport. Ref.10 Ref.11 Ref.13 Ref.14
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: Serine/threonine-protein kinase activity is promoted by associated cyclins CCDN3 and CCNY and repressed by CDKN1A.
Subunit structure: Interacts with CCNY; CCNY mediates its recruitment to the plasma membrane and promotes phosphorylation of LRP6. Interacts with CCDN3 and CDKN1A. Interacts with SEPT8. Interacts with 14-3-3 proteina YWHAB, YWHAE, YWHAH and YWHAQ. Ref.8 Ref.9 Ref.11 Ref.13 Ref.14
Subcellular location: Cell membrane; Peripheral membrane protein. Cytoplasm. Nucleus. Note: Recruited to the cell membrane by CCNY. Ref.1 Ref.13 Ref.14
Tissue specificity: Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected. Ref.1
Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.Contains 1 protein kinase domain.
Sequence caution: The sequence BAA74857.2 differs from that shown. Reason: Erroneous initiation.
Research Articles on PFTK1 (CDK14)
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Product Notes
The PFTK1 (CDK14) cdk14 (Catalog #AAA515112) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human PFTK1 (CDK14) was expressed by baculovirus in Sf9 insect cells. AAA Biotech's PFTK1 (CDK14) can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the PFTK1 (CDK14) cdk14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFTK1 (CDK14), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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