Monkey CDK9 ELISA Kit | CDK-9 elisa kit

Monkey CDK9 (Cyclin Dependent Kinase 9) ELISA Kit

Cat. Num. AAA2509147

• Gene Names: CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
• Reactivity: Monkey
• Synonyms: CDK9; Monkey CDK9 (Cyclin Dependent Kinase 9) ELISA Kit; CDK-9 elisa kit

• Reactivity: Monkey
• Specificity: This kit recognizes natural and recombinant Monkey CDK-9. No significant cross-reactivity or interference between Monkey CDK-9 and analogues was observed.
• Sequence Length: 372

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for CDK-9 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Monkey CDK-9 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK-9. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CDK-9 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CDK-9. You can calculate the concentration of CDK-9 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502747
• NCBI GeneID: 1025
• NCBI Accession #: NP_001252.1
• NCBI GenBank Nucleotide #: NM_001261.3
• UniProt Accession #: P50750; Q5JU24; Q5JU25; Q5U006; Q96TF1
• Molecular Weight: 53,365 Da
• NCBI Official Full Name: cyclin-dependent kinase 9
• NCBI Official Synonym Full Names: cyclin-dependent kinase 9
• NCBI Official Symbol: CDK9
• NCBI Official Synonym Symbols: TAK; C-2k; CTK1; CDC2L4; PITALRE
• NCBI Protein Information: cyclin-dependent kinase 9; CDC2-related kinase; cell division cycle 2-like protein kinase 4; cell division protein kinase 9; serine/threonine protein kinase PITALRE; tat-associated kinase complex catalytic subunit
• UniProt Protein Name: Cyclin-dependent kinase 9
• Protein Family: Probable cyclin-dependent kinase
• UniProt Gene Name: CDK9
• UniProt Synonym Gene Names: CDC2L4; TAK
• UniProt Entry Name: CDK9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK9: a protein kinase of the CDK family. Forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. A component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. Transcriptional elongation factor and cofactor for HIV Tat protein; RNAi blocks HIV replication, and inhibitors also block varicella zoster replication. Mediates signals leading to cardiac hypertrophy. Inhibitor: Flavopiridol.

Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; EC 2.7.11.22; EC 2.7.11.23; Nuclear receptor co-regulator; CMGC group; CDK family; CDK9 subfamily; CDK/CDK9 subfamily

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; transcription elongation factor complex; PML body; transcription elongation factor complex b; intracellular membrane-bound organelle; membrane; cytoplasm

Molecular Function: RNA polymerase subunit kinase activity; protein binding; snRNA binding; DNA binding; cyclin-dependent protein kinase activity; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of histone modification; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; viral reproduction; positive regulation of histone phosphorylation; positive regulation of viral transcription; regulation of mitotic cell cycle; DNA repair; protein amino acid phosphorylation; regulation of DNA repair; cell proliferation; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; replication fork arrest; positive regulation of transcription from RNA polymerase II promoter