R-Cadherin/CDH4 Recombinant Protein | CDH4 recombinant protein

R-Cadherin/CDH4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120121

• Gene Names: CDH4; CAD4; RCAD; R-CAD
• Purity: >85% as determined by SDS-PAGE
• Synonyms: R-Cadherin/CDH4; R-Cadherin/CDH4 Protein; Human; Recombinant (His Tag); Human R-Cadherin/CDH4 Protein (His Tag); CAD4 Protein; R-CAD Protein; RCAD Protein; cadherin 4; type 1; R-cadherin (retinal); CDH4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala734

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of human CAD4 (NP_001785.2)(Met 1-Ala 734) was expressed with a fused polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDH4 recombinant protein

Background: The cadherin superfamily is a large family that engage in both homo- and heterotypic, calcium-dependent, cell-cell adhesion events, and can be divided into at least four subfamilies based on the extracellular (EC) regions and cytoplasmic domains, that is: classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules. Human cadherin 4, type 1, R-cadherin (retinal), also known as CDH4, CAD4 and RCAD, is a classical cadherin from the cadherin superfamily. It is a calcium-dependent adhesion molecule and a type I transmembrane glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. CDH4 is thought to play an important role during brain segmentation and neuronal outgrowth, and also exerts critical actions in kidney and muscle development. CDH4 is expressed in vascular smooth muscle, pancreatic beta-cells, thyroid follicular cells, sensory neurons of the dorsal root ganglia, and, possibly, astrocytes and endothelium of the retina. As a classic cadherin, CDH4 forms both homodimers and heterodimers with N-cadherin. The extracellular region of human CDH4 is 96% aa identical to that of mouse CDH4.

Product Categories/Family for CDH4 recombinant protein

Canonical Wnt Pathway

References

Suzuki, S. et al., 1991, Cell Regul. 2: 261-270. Matsunami, H. et al., 1993, J. Cell Sci. 106: 401-409. Tanihara, H. et al.,1995, Cell Adhes. Commun. 2 (1): 15-26. Shan, W-S., et al., 2000, J. Cell Biol. 148: 579-590. Fagman, H. et al, 2003, Endocrinology. 144: 3618-3624. Koch, A.W. et al., 2004, Cell. Mol. Life Sci. 61: 1884-1895.

NCBI and Uniprot Product Information

• NCBI GI #: 14589893
• NCBI GeneID: 1002
• NCBI Accession #: NP_001785.2
• NCBI GenBank Nucleotide #: NM_001794.4
• UniProt Accession #: P55283; Q2M208; Q5VZ44; Q9BZ05; B3KWB8; G3V1P8
• Molecular Weight: 92,388 Da
• NCBI Official Full Name: cadherin-4 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cadherin 4, type 1, R-cadherin (retinal)
• NCBI Official Symbol: CDH4
• NCBI Official Synonym Symbols: CAD4; RCAD; R-CAD
• NCBI Protein Information: cadherin-4; R-cadherin; retinal cadherin; cadherin 4, type 1, preproprotein
• UniProt Protein Name: Cadherin-4
• UniProt Gene Name: CDH4
• UniProt Synonym Gene Names: R-CAD; R-cadherin
• UniProt Entry Name: CADH4_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

CDH4: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development. Expressed mainly in brain but also found in other tissues.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: heterophilic cell adhesion; axon guidance; intercellular junction assembly and maintenance; positive regulation of axon extension; cell adhesion; homophilic cell adhesion