Cadherin-1 Recombinant Protein | CDH1 recombinant protein

Cadherin-1, 24-709aa, Human, His tag, Baculovirus

Cat. Num. AAA206233

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Cadherin-1; 24-709aa; Human; His tag; Baculovirus; CDH1; Arc-1; CD324; CDHE; ECAD; LCAM; UVO; CDH1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: EPEPCHPGFD AESYTFTVPR RHLERGRVLG RVNFEDCTGR QRTAYFSLDT RFKVGTDGVI TVKRPLRFHN PQIHFLVYAW DSTYRKFSTK VTLNTVGHHH RPPPHQASVS GIQAELLTFP NSSPGLRRQK RDWVIPPISC PENEKGPFPK NLVQIKSNKD KEGKVFYSIT GQGADTPPVG VFIIERETGW LKVTEPLDRE RIATYTLFSH AVSSNGNAVE DPMEILITVT DQNDNKPEFT QEVFKGSVME GALPGTSVME VTATDADDDV NTYNAAIAYT ILSQDPELPD KNMFTINRNT GVISVVTTGL DRESFPTYTL VVQAADLQGE GLSTTATAVI TVTDTNDNPP IFNPTTYKGQ VPENEANVVI TTLKVTDADA PNTPAWEAVY TILNDDGGQF VVTTNPVNND GILKTAKGLD FEAKQQYILH VAVTNVVPFE VSLTTSTATV TVDVLDVNEA PIFVPPEKRV EVSEDFGVGQ EITSYTAQEP DTFMEQKITY RIWRDTANWL EINPDTGAIS TRAELDREDF EHVKNSTYTA LIIATDNGSP VATGTGTLLL ILSDVNDNAP IPEPRTIFFC ERNPKPQVIN IIDADLPPNT SPFTAELTHG ASANWTIQYN DPTQESIILK PKMALEVGDY KINLKLMDNQ NKDQVTTLEV SVCDCEGAAG VCRKAQPVEA GLQIPALEHH HHHH
• Sequence Length: 882

• Applicable Applications for CDH1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CDH1 recombinant protein

CDH1, also known as cadherin-1, is a member of cell surface glycoproteins that meditate cell adhesion. Human CDH1 shares amino acid sequence identity with the rat and mouse proteins. It is a single-pass transmembrane protein that meditates calcium-dependent epithelial cell adhesion. This protein preferentially interacts with themselves in a homophilic manner in connecting cells. It may thus contribute to the sorting of heterogeneous cell types. It is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. It is a ligand for integrin alpha-E/beta-7. Recombinanat human CDH1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CDH1 recombinant protein

Signal Transduction; Cytoskeleton; Adhesion & Movement

References

Fulga V., et al. (2015) Anticancer Res. 35:759-765.; Zhang L., el al. (2014) Mutat. Res. 770:106-111.

NCBI and Uniprot Product Information

• NCBI GI #: 4757960
• NCBI GeneID: 999
• NCBI Accession #: NP_004351
• NCBI GenBank Nucleotide #: NM_004360.4
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• Molecular Weight: 76.6kDa (694aa) 70KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: cadherin-1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Uniprot Description

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.