CDC42 recombinant protein

CDC42, Active

Cat. Num. AAA515462

• Gene Names: CDC42; TKS; G25K; CDC42Hs
• Applications: Western Blot
• Synonyms: CDC42; Active; CDC42 recombinant protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full length
• Sequence Length: 2182

• Applicable Applications for CDC42 recombinant protein: Enzyme Assay, Western Blot (WB)
• Species: Human
• Tag: GST/HIS
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for CDC42 recombinant protein

Description: Recombinant full length human CDC42 was expressed in E Coli cells using an N-dual GST/His tag.
Scientific Background: CDC42 is a member of the Rho family of GTPases and is part of cellular pathways fundamental to growth, differentiation and apoptosis (1). Downstream targets for CDC42 include those that regulate the actin cytoskeleton (e.g. WASP) and cellular stress pathways (e.g. PAK) as well as the coatomer protein complex and PAR6. CDC42 is involved in the G (1)-S progression of the cell cycle. CDC42 and its downstream effector mDia3 are involved in bi-orientation and stabilization of spindle microtubules attachment to kinetochores and regulate chromosome alignment and segregation during mitosis (2).

Product Categories/Family for CDC42 recombinant protein

Enzymes; Active GTPases/ATPases

References

1. Erickson, J.W. et al: Multiple roles for Cdc42 in cell regulation. Curr Opin Cell Biol. 2001 Apr;13 (2):153-7. 2. Narumyia, S. et al: A new look at Rho GTPases in cell cycle: role in kinetochore-microtubule attachment. Cell Cycle. 2004 Jul;3 (7):855-7.

NCBI and Uniprot Product Information

• NCBI GI #: 89903013
• NCBI GeneID: 998
• NCBI Accession #: NM_001791.3
• NCBI GenBank Nucleotide #: NM_001791
• UniProt Accession #: P60953; P21181; P25763; Q7L8R5; Q9UDI2
• Molecular Weight: ~48 kDa
• NCBI Official Full Name: Homo sapiens cell division cycle 42 (CDC42), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: cell division cycle 42
• NCBI Official Symbol: CDC42
• NCBI Official Synonym Symbols: TKS; G25K; CDC42Hs
• NCBI Protein Information: cell division control protein 42 homolog
• UniProt Protein Name: Cell division control protein 42 homolog
• Protein Family: Cdc42
• UniProt Gene Name: CDC42

NCBI Description

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

Uniprot Description

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation ().