Bovine Cell division control protein 42 ELISA Kit | CDC42 elisa kit

Bovine Cell division control protein 42 homolog ELISA Kit

Cat. Num. AAA2887368

• Reactivity: Bovine
• Synonyms: Cell division control protein 42; Bovine Cell division control protein 42 homolog ELISA Kit; Cell division control protein 42 homolog; CDC42; CDC42 elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural BovineCell division control protein 42 homolog. No significant cross-reactivity or interference was observed.
• Sequence Length: 191

• Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Assay Type: Quantitative Competitive
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDC42 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovineCell division control protein 42 homologconcentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toCell division control protein 42 homolog, During the reaction,Cell division control protein 42 homologin the sample or standard competes with a fixed amount of biotin-labeledCell division control protein 42 homolog for sites on a pre-coated Monoclonal antibody specific to Cell division control protein 42 homolog. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cell division control protein 42 homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 114052486
• NCBI GeneID: 532712
• NCBI Accession #: NP_001039797.1
• NCBI GenBank Nucleotide #: NM_001046332.1
• UniProt Accession #: Q2KJ93
• Molecular Weight: 21,311 Da
• NCBI Official Full Name: cell division control protein 42 homolog
• NCBI Official Symbol: CDC42
• NCBI Protein Information: cell division control protein 42 homolog
• UniProt Protein Name: Cell division control protein 42 homolog
• Protein Family: Cdc42
• UniProt Gene Name: CDC42
• UniProt Entry Name: CDC42_BOVIN

Uniprot Description

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia ().