Cluster Of Differentiation 8b (CD8b) Recombinant Protein | CD8b recombinant protein

Recombinant Cluster Of Differentiation 8b (CD8b)

Cat. Num. AAA2018826

• Gene Names: CD8B; LY3; P37; LEU2; LYT3; CD8B1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cluster Of Differentiation 8b (CD8b); Recombinant Cluster Of Differentiation 8b (CD8b); CD8b recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LQQTPAYIK VQTNKMVMLS CEAKISLSNM RIYWLRQRQA PSSDSHHEFL ALWDSAKGTI HGEEVEQEKI AVFRDASRFI LNLTSVKPED SGIYFCMIVG SPELTFGKGT QLSVVDFLPT TAQPTKKSTL KKRVCRLPRPETQKGPLCSP
• Sequence Length: 198

• Applicable Applications for CD8b recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu22~Pro170 (Accession # P10966) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein. Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 296010933
• NCBI GeneID: 926
• NCBI Accession #: NP_001171571.1
• NCBI GenBank Nucleotide #: NM_001178100.1
• UniProt Accession #: P10966; P14860; P14861; Q15980; Q496E0; Q496E1; Q496E2; Q9UDB4; Q9UDB5; Q9UDB6; Q9UDB7; Q9UDB8
• Molecular Weight: 20.6kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD8 beta chain isoform 6
• NCBI Official Synonym Full Names: CD8b molecule
• NCBI Official Symbol: CD8B
• NCBI Official Synonym Symbols: LY3; P37; LEU2; LYT3; CD8B1
• NCBI Protein Information: T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 beta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8B
• UniProt Synonym Gene Names: CD8B1
• UniProt Entry Name: CD8B_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]

Uniprot Description

CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: T cell receptor complex; early endosome membrane; integral to plasma membrane; extracellular region; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; MHC class I protein binding; coreceptor activity

Biological Process: regulation of immune response; viral reproduction; T cell activation; immune response; regulation of defense response to virus by virus; transmembrane receptor protein tyrosine kinase signaling pathway