CD74 recombinant protein

CD74, human recombinant

Cat. Num. AAA845075

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: CD74; human recombinant; HLA class II histocompatibility antigen gamma chain isoform b; DHLAG; HLADG; Ia-GAMMA; II; CD74 recombinant protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 1 mM DTT and 30% glycerol.; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSQQQGRLD KLTVTSQNLQ LENLRMKLPK PPKPVSKMRM ATPLLMQALP MGALPQGPMQ NATKYGNMTE DHVMHLLQNA DPLKVYPPLK GSFPENLRHL KNTMETIDWK VFESWMHHWL LFEMSRHSLE QKPTDAPPKE SLELEDPSSG LGVTKQDLGP VPM
• Sequence Length: 160

• Applicable Applications for CD74 recombinant protein: SDS-PAGE
• Endotoxin Level: < 1.0 EU per 1 microgram of protein
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CD74 recombinant protein

Background: HLA class II histocompatibility antigen gamma chain isoform b, also known as CD74, is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. It is upregulated in several cancers and is also expressed by nonimmune cells during inflammation. Recombinant human CD74 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family for CD74 recombinant protein

Cytokines; Growth Factors & Hormones; CDsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth F

NCBI and Uniprot Product Information

• NCBI GI #: 68448537
• NCBI GeneID: 972
• NCBI Accession #: NP_001020329.1
• NCBI GenBank Nucleotide #: NM_001025158.2
• UniProt Accession #: P04233; Q14597; Q29832; Q5U0J8; Q8SNA0; Q8WLP6; A8K7R1; B4DNE8; D3DQG3; D3DQG4
• Molecular Weight: 20.6 kDa (183 aa, 73-232 aa + His tag), confirmed by MALDI-TOF.
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform c
• NCBI Official Synonym Full Names: CD74 molecule, major histocompatibility complex, class II invariant chain
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii
• UniProt Entry Name: HG2A_HUMAN

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

CD74: Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74- ROS1 chimeric protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: Golgi membrane; multivesicular body; lysosomal lumen; cell surface; membrane; lysosomal membrane; integral to membrane; plasma membrane; trans-Golgi network membrane; vacuole; intracellular; external side of plasma membrane; MHC class II protein complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; cytokine binding; beta-amyloid binding; MHC class II protein binding, via antigen binding groove; nitric-oxide synthase binding; MHC class II protein binding

Biological Process: negative regulation of peptide secretion; positive regulation of T-helper 2 type immune response; chaperone cofactor-dependent protein folding; activation of MAPK activity; positive regulation of dendritic cell antigen processing and presentation; regulation of macrophage activation; T cell selection; antigen processing and presentation of exogenous peptide antigen via MHC class II; defense response; prostaglandin biosynthetic process; signal transduction; negative thymic T cell selection; negative regulation of T cell differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell proliferation; intracellular protein transport; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; positive regulation of T cell differentiation; positive regulation of B cell proliferation; protein complex assembly; immunoglobulin mediated immune response; positive regulation of cytokine and chemokine mediated signaling pathway; antigen processing and presentation of endogenous antigen; negative regulation of apoptosis; positive thymic T cell selection