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Human Cluster of differentiation 63 ELISA Kit | CD63 elisa kit

Human Cluster of differentiation 63 (CD63) ELISA Kit

Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human
Synonyms
Cluster of differentiation 63; Human Cluster of differentiation 63 (CD63) ELISA Kit; CD63; LAMP-3; ME491; MLA1; OMA81H; Melanoma 1 antigen; Granulophysin; Lysosomal-associated membrane protein 3; Ocular melanoma-associated antigen; CD63 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CD63. No significant cross-reactivity or interference between Human CD63 and analogues was observed.
Sequence Length
245
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for CD63 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CD63 in samples. An antibody specific for CD63 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD63 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD63 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD63 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
967
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,271 Da
NCBI Official Full Name
CD63 antigen isoform A
NCBI Official Synonym Full Names
CD63 molecule
NCBI Official Symbol
CD63
NCBI Official Synonym Symbols
MLA1; ME491; LAMP-3; OMA81H; TSPAN30
NCBI Protein Information
CD63 antigen
UniProt Protein Name
CD63 antigen
Protein Family
UniProt Gene Name
CD63
UniProt Synonym Gene Names
MLA1; TSPAN30; LAMP-3; Tspan-30

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

CD63: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: cell surface; endosome membrane; extracellular space; integral component of plasma membrane; intrinsic component of plasma membrane; late endosome membrane; lysosomal membrane; melanosome; plasma membrane; platelet dense granule membrane

Molecular Function: protein binding

Biological Process: cell migration; cell-matrix adhesion; neutrophil degranulation; pigment granule maturation; platelet degranulation; positive regulation of receptor internalization; protein transport

Research Articles on CD63

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Product Notes

The Human CD63 cd63 (Catalog #AAA284221) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284221 ELISA Kit recognizes Human CD63. It is sometimes possible for the material contained within the vial of "Cluster of differentiation 63, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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