Human Cluster Of differentiation ELISA Kit | CD44 elisa kit
Human Cluster Of differentiation (CD44) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]
Uniprot Description
CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: apical plasma membrane; cell projection; cell surface; cytosol; extracellular exosome; focal adhesion; Golgi apparatus; integral component of plasma membrane; plasma membrane; secretory granule membrane
Molecular Function: collagen binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; hyaluronic acid binding; protein binding
Biological Process: cartilage development; cell-matrix adhesion; cellular response to fibroblast growth factor stimulus; extracellular matrix disassembly; extracellular matrix organization; hyaluronan catabolic process; leukocyte migration; negative regulation of apoptosis; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; neutrophil degranulation; positive regulation of ERK1 and ERK2 cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of monocyte aggregation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of lamellipodium morphogenesis; wound healing, spreading of cells
Disease: Blood Group, Indian System
