CD3D & CD3E Heterodimer Recombinant Protein | CD3D&CD3E recombinant protein

CD3D & CD3E Heterodimer Protein, Mouse, Recombinant

Cat. Num. AAA8123611

• Gene Names: Cd3d; T3d
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CD3D & CD3E Heterodimer; CD3D & CD3E Heterodimer Protein; Mouse; Recombinant; Mouse CD3D & CD3E Heterodimer Protein; T3d Protein; CD3d molecule; delta (CD3-TCR complex); CD3D&CD3E recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala105&Met1-Asp108

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 22 & Asp 22
• Tag: C-human IgG1-Fc & DYKDDDDK & C-human IgG1-Fc & His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal DYKDDDDK-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 227498961
• NCBI GeneID: 12500
• NCBI Accession #: NP_038515.3
• NCBI GenBank Nucleotide #: NM_013487.3
• UniProt Accession #: P04235; P22646
• Molecular Weight: 19,032 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 delta chain
• NCBI Official Synonym Full Names: CD3 antigen, delta polypeptide
• NCBI Official Symbol: Cd3d
• NCBI Official Synonym Symbols: T3d
• NCBI Protein Information: T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 delta chain
• UniProt Gene Name: Cd3d
• UniProt Synonym Gene Names: T3d
• UniProt Entry Name: CD3D_MOUSE

Uniprot Description

CD3D: a T cell surface glycoprotein that is a component of the T cell antigen receptor. A defect in this protein causes a severe combined immunodeficiency characterized by the absence of T cells but normal B cells. Contains 1 ITAM domain.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: T cell receptor complex; membrane; integral to membrane; alpha-beta T cell receptor complex

Molecular Function: protein binding; transmembrane receptor activity; protein heterodimerization activity

Biological Process: cell surface receptor linked signal transduction; T cell activation; protein complex assembly; positive thymic T cell selection