Cyclic ADP Ribose Hydrolase Recombinant Protein | cADPRH recombinant protein

Recombinant Cyclic ADP Ribose Hydrolase (cADPRH)

Cat. Num. AAA2031477

• Gene Names: CD38; T10; ADPRC 1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cyclic ADP Ribose Hydrolase; Recombinant Cyclic ADP Ribose Hydrolase (cADPRH); cADPRH recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.6 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KGKPTTKH FADIILGRCL IYTQILRPEM RDQDCKKILS TFKRGFISKN PCNITNEDYA PLVKLVTQTI PCNKTLFWSK SKHLAHQYTW IQGKMFTLED TLLGYIADDL RWCGDPSTSD MNYDSCPHWS ENCPNNPVAV FWNVISQKFA EDACGVVQVM LNGSLSEPFY RNSTFGSVEV FNLDPNKVHK LQAWVMHDIKGTSSNACSSP SINELKSIVN KRNMIFACQD NYRP
• Sequence Length: 60

• Applicable Applications for cADPRH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.3kDa
• Accurate Molecular Mass (KD): 30kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Glu510~Thr690
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 68989438
• NCBI GeneID: 952
• Molecular Weight: 13,788 Da
• NCBI Official Full Name: cyclic ADP-ribose hydrolase, partial
• NCBI Official Synonym Full Names: CD38 molecule
• NCBI Official Symbol: CD38
• NCBI Official Synonym Symbols: T10; ADPRC 1
• NCBI Protein Information: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Protein Name: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
• UniProt Gene Name: CD38
• UniProt Synonym Gene Names: ADPRC 1; cADPr hydrolase 1
• UniProt Entry Name: CD38_HUMAN

NCBI Description

The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

CD38: Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system. Belongs to the ADP-ribosyl cyclase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Hydrolase; Membrane protein, integral; Lyase; Apoptosis; EC 3.2.2.6; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cell surface; EC 2.4.99.20

Chromosomal Location of Human Ortholog: 4p15

Cellular Component: cell surface; membrane; integral to membrane; plasma membrane; nucleus

Molecular Function: transferase activity; phosphorus-oxygen lyase activity; NAD+ nucleosidase activity; NAD(P)+ nucleosidase activity

Biological Process: response to drug; response to retinoic acid; metabolic process; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; female pregnancy; signal transduction; positive regulation of cell growth; response to estradiol stimulus; B cell receptor signaling pathway; elevation of cytosolic calcium ion concentration; response to hydroperoxide; positive regulation of B cell proliferation; positive regulation of vasoconstriction; response to hypoxia; negative regulation of transcription, DNA-dependent; negative regulation of bone resorption; response to progesterone stimulus; negative regulation of apoptosis