CD34 recombinant protein

CD34, Recombinant, Human (Endothelial Cell Marker, Cluster designation 34, Hematopoietic progenitor cell antigen CD34, HPCA1)

Cat. Num. AAA635968

• Applications: ELISA, Western Blot
• Purity: Highly Purified; Purified
• Synonyms: CD34; Recombinant; Human (Endothelial Cell Marker; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1); CD34 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; Purified
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 10mM L-glutathione (reduced).

• Applicable Applications for CD34 recombinant protein: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Inhibition Assays and Western Blot.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CD34 recombinant protein

CD34 is a cell surface antigen formerly known as hemopoietic progenitor cell antigen-1. CD34 is expressed selectively on human hematopoietic progenitor cells. The biological function of CD34 is still unknown. It is used a surface marker for very early hematopoietic stem cells. Cells expressing CD34 respond to SCF (stem cell factor) and LIF in the presence of colony stimulating factors. These factors can induce myeloid differentiation in these cells. The effects of some growth factors on cells expressing CD34 are counteracted by IP-10, a factor the expression of which is induced by IFN-gamma. The proliferation of human CD34 cells is stimulated also by IL-11, and IL-11 synergizes with SCF, IL-3, and GM-CSF. In the presence of human IL-17, fibroblasts can sustain the proliferation of CD34 hematopoietic progenitors and their preferential maturation into neutrophils.

Recombinant CD34, a 385 amino acid long protein, shows a band at 40kD on SDS-PAGE commassie blue stained gel.

Product Categories/Family for CD34 recombinant protein

Molecular Biology; MB-CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 264769
• NCBI GeneID: 947
• UniProt Accession #: P28906; Q15970; Q15971; Q5JTA3; Q5JTA4; Q9UJB1; A8K664
• Molecular Weight: 40kD
• NCBI Official Full Name: CD34
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34; CD34 antigen
• UniProt Protein Name: Hematopoietic progenitor cell antigen CD34
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: CD34
• UniProt Entry Name: CD34_HUMAN

NCBI Description

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; integral to plasma membrane; lysosome; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; plasma membrane; basal plasma membrane; external side of plasma membrane

Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; cell-cell adhesion; positive regulation of angiogenesis; regulation of blood pressure; endothelial cell proliferation; tissue homeostasis; hemopoiesis; leukocyte migration