CD28 recombinant protein

CD28 Protein, Mouse, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8122530

• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD28; CD28 Protein; Mouse; Recombinant (ECD; hFc Tag); Mouse CD28/TP44 Protein (Fc Tag); CD28 molecule; CD28 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys149

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Asn 20
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse CD28 (NP_031668.3)(Met1-Lys149) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD28 recombinant protein

Background: CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Product Categories/Family for CD28 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets; T Cell Receptor Signaling Pathway

References

Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.

NCBI and Uniprot Product Information

• NCBI GI #: 161484597
• NCBI GeneID: 12487
• NCBI Accession #: NP_031668.3
• NCBI GenBank Nucleotide #: NM_007642.4
• Molecular Weight: 25,243 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein