CD226 recombinant protein

CD226, 19-247aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206482

• Gene Names: CD226; PTA1; DNAM1; DNAM-1; TLiSA1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CD226; 19-247aa; Human; hIgG-His-tag; Baculovirus; CD226 antigen isoform; DNAM-1; DNAM1; PTA1; TLiSA1; CD226 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPEEVLWHT SVPFAENMSL ECVYPSMGIL TQVEWFKIGT QQDSIAIFSP THGMVIRKPY AERVYFLNST MASNNMTLFF RNASEDDVGY YSCSLYTYPQ GTWQKVIQVV QSDSFEAAVP SNSHIVSEPG KNVTLTCQPQ MTWPVQAVRW EKIQPRQIDL LTYCNLVHGR NFTSKFPRQI VSNCSHGRWS VIVIPDVTVS DSGLYRCYLQ ASAGENETFV MRLTVAEGKT DNLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H
• Sequence Length: 336

• Applicable Applications for CD226 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CD226 recombinant protein

CD226, also known as CD226 antigen isoform, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. It is an activating receptor expressed on NK cells and T cells and plays an important role in cytotoxicity of these cells against target cells. It mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells. Recombinant human CD226, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CD226 recombinant protein

Immunology

References

Vo AV., et al. (2016) Mol Immunol. 69:70-76.; Kojima H., et al. (2003) J Biol Chem. 278:36748-36753.

NCBI and Uniprot Product Information

• NCBI GI #: 171460997
• NCBI GeneID: 10666
• NCBI Accession #: NP_006557
• NCBI GenBank Nucleotide #: NM_006566.3
• UniProt Accession #: Q15762; B2R818
• Molecular Weight: 53.2kDa (471aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: CD226 antigen isoform a
• NCBI Official Synonym Full Names: CD226 molecule
• NCBI Official Symbol: CD226
• NCBI Official Synonym Symbols: PTA1; DNAM1; DNAM-1; TLiSA1
• NCBI Protein Information: CD226 antigen
• UniProt Protein Name: CD226 antigen
• Protein Family: CD226 antigen
• UniProt Gene Name: CD226
• UniProt Synonym Gene Names: DNAM1; DNAM-1

NCBI Description

This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704). Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705).