CD1D & B2M Heterodimer Recombinant Protein | CD1D&B2M recombinant protein

CD1D & B2M Heterodimer Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8123614

• Gene Names: CD1D; R3; CD1A
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD1D & B2M Heterodimer; CD1D & B2M Heterodimer Protein; Human; Recombinant (His Tag); Human CD1D & B2M Heterodimer Protein; CD1D&B2M recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser301&Met1-Met119

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 20 & Ile 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met1-Ser301) of human CD1D (P15813) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769)(Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the CD1D/B2M heterodimer was purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 115964
• NCBI GeneID: 912
• UniProt Accession #: P15813; P61769
• Molecular Weight: 335
• NCBI Official Full Name: Antigen-presenting glycoprotein CD1d
• NCBI Official Synonym Full Names: CD1d molecule
• NCBI Official Symbol: CD1D
• NCBI Official Synonym Symbols: R3; CD1A
• NCBI Protein Information: antigen-presenting glycoprotein CD1d; R3G1; thymocyte antigen CD1D; CD1D antigen, d polypeptide; T-cell surface glycoprotein CD1d; differentiation antigen CD1-alpha-3; HMC class I antigen-like glycoprotein CD1D
• UniProt Protein Name: Antigen-presenting glycoprotein CD1d
• UniProt Gene Name: CD1D
• UniProt Entry Name: CD1D_HUMAN

NCBI Description

This gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

CD1D: Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

Protein type: Apoptosis; Membrane protein, integral; Lipid-binding; Cell surface

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cell surface; lysosomal membrane; integral to plasma membrane; cytoplasm; endosome membrane

Molecular Function: histone binding; beta-2-microglobulin binding; exogenous lipid antigen binding; lipid antigen binding; cell adhesion molecule binding; receptor activity; endogenous lipid antigen binding

Biological Process: antigen processing and presentation, endogenous lipid antigen via MHC class Ib; heterotypic cell-cell adhesion; detection of bacterium; viral reproduction; positive regulation of innate immune response; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; T cell selection; innate immune response; positive regulation of T cell proliferation