Typical Testing Data/Standard Curve (for reference only)
Human Cluster of differentiation 19 ELISA Kit | CD19 elisa kit
Human Cluster of differentiation 19 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This CD19 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD19. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CD19 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD19. Standards or samples are then added to the microtiter plate wells and CD19 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD19 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD19 are added to each well to “sandwich” the CD19 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD19 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD19 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
Uniprot Description
CD19: a cell surface molecule which assembles with the antigen receptor of B lymphocytes. A critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation. Ligation increases intracellular calcium levels and decreases the threshold for antigen receptor signaling. Germinal center formation is significantly reduced in CD19-deficient mice.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: protein complex; integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: receptor signaling protein activity
Biological Process: epidermal growth factor receptor signaling pathway; B cell receptor signaling pathway; regulation of immune response; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cell surface receptor linked signal transduction; nerve growth factor receptor signaling pathway; cellular defense response; innate immune response; positive regulation of release of sequestered calcium ion into cytosol
Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 3
Research Articles on CD19
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Product Notes
The Human CD19 cd19 (Catalog #AAA723885) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA723885 ELISA Kit recognizes Human CD19. It is sometimes possible for the material contained within the vial of "Cluster of differentiation 19, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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