PRV1/CD177 recombinant protein

Recombinant Human PRV1/CD177 Protein (C-6His)

Cat. Num. AAA2553166

• Gene Names: CD177; NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: PRV1/CD177; Recombinant Human PRV1/CD177 Protein (C-6His); CD177 Antigen; Human Neutrophil Alloantigen 2a; HNA-2a; NB1 Glycoprotein; NB1 GP; Polycythemia Rubra Vera Protein 1; PRV-1; CD177; NB1; PRV1; PRV1/CD177 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence Positions: Leu22-Gly407
• Sequence Length: 437

• Species: Human
• Endotoxin: <1.0 EU per ug as determined by LAL test.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for PRV1/CD177 recombinant protein

CD177 is polymorphic and has at least two alleles: PRV1 and NB1. Human PRV1 is a Glycosyl-Phosphatidylinositol (GPI)-linked cell surface glycoprotein that belongs to the uPAR/CD59/Ly6 family of receptors. PRV1 is expressed by neutrophils and neutrophil precursors, and changes in expression serve as markers for myeloproliferative disorders such as polycythemia vera and essential thrombocythemia. PRV1 may also be expressed by Erythroblasts, B cells, and Monocytes. NB1, a Glycosyl-Phosphatidylinositol (GPI)-linked cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia. It is reported that CD177 functions as a novel heterophilic binding partner that engages PECAM-1 in membrane-proximal IgD6.

NCBI and Uniprot Product Information

• NCBI GI #: 110735433
• NCBI GeneID: 57126
• NCBI Accession #: NP_065139.2
• NCBI GenBank Nucleotide #: NM_020406.3
• UniProt Accession #: Q8N6Q3; Q711Q2; Q8NCV9; Q96QH1; Q9HDA5
• Molecular Weight: Molecular Mass: 42.3 kDa; Actual Protein Molecular Mass: 55 kDa
• NCBI Official Full Name: CD177 antigen
• NCBI Official Synonym Full Names: CD177 molecule
• NCBI Official Symbol: CD177
• NCBI Official Synonym Symbols: NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1 GP
• NCBI Protein Information: CD177 antigen
• UniProt Protein Name: CD177 antigen
• UniProt Gene Name: CD177
• UniProt Synonym Gene Names: NB1; PRV11 PublicationManual assertion based on opinion iniRef.1; PRV-11 PublicationManual assertion based on opinion iniRef.1

NCBI Description

This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified. [provided by RefSeq, Apr 2014]

Uniprot Description

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).