CD172a/SIRPA recombinant protein

Recombinant Human CD172a/SIRPA Protein (C-His)

Cat. Num. AAA2557134

• Gene Names: SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: CD172a/SIRPA; Recombinant Human CD172a/SIRPA Protein (C-His); Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; SHP Substrate 1; SHPS-1; Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs; Bit; CD172 Antigen-Like Family Member A; Inhibitory Feceptor SHPS-1; Macrophage Fusion Receptor; MyD-1 Antigen; Signal-Regulatory Protein Alpha-1; Sirp-Alpha-1; Signal-Regulatory Protein Alpha-2; Sirp-Alpha-2; Signal-Regulatory Protein Alpha-3; Sirp-Alpha-3; p84; CD172a; SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; CD172a/SIRPA recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: Glu31-Arg370
• Sequence Length: 503

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for CD172a/SIRPA recombinant protein

Protein Construction: Recombinant Human CD172a is produced by our mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a 6His tag at the C-terminus.

Background: Signal Regulatory Protein alpha (SIRPalpha) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPalpha contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPalpha can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPalpha acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPalpha shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPalpha engagement generally produces a negative regulatory signal; it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation

NCBI and Uniprot Product Information

• NCBI GI #: 2052056
• NCBI GeneID: 140885
• Molecular Weight: Molecular Mass: 38.31kDa; Actual Protein Molecular Mass: 45-65kDa
• NCBI Official Full Name: SIRP-alpha1
• NCBI Official Synonym Full Names: signal regulatory protein alpha
• NCBI Official Symbol: SIRPA
• NCBI Official Synonym Symbols: BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]