CD14 Recombinant Protein | mCD14 recombinant protein

Recombinant Mouse CD14

Cat. Num. AAA143088

• Applications: ELISA, Western Blot
• Synonyms: CD14; Recombinant Mouse CD14; CD14 Mouse; CD14 Mouse Recombinant; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; mCD14 recombinant protein

• Host: Chinese Hamster Ovarian Cells (CHO)
• Form/Format: CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence Length: 366

• Applicable Applications for mCD14 recombinant protein: ELISA (EIA), Inhibition Assays, Western Blot (WB)
• Solubility: It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Mouse CD14 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mCD14 recombinant protein

Description: The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controlled by SDS page.The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.

Introduction: CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

Product Categories/Family for mCD14 recombinant protein

Recombinant & Natural Proteins; CD Antigens

NCBI and Uniprot Product Information

• NCBI GI #: 6753332
• NCBI GeneID: 12475
• NCBI Accession #: NP_033971.1
• NCBI GenBank Nucleotide #: NM_009841.4
• UniProt Accession #: P10810
• Molecular Weight: 39,204 Da
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 antigen
• NCBI Official Symbol: Cd14
• NCBI Protein Information: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
• UniProt Protein Name: Monocyte differentiation antigen CD14
• UniProt Gene Name: Cd14
• UniProt Entry Name: CD14_MOUSE

NCBI Description

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]

Uniprot Description

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: extracellular space; cell surface; membrane; plasma membrane; lipid raft

Molecular Function: lipopolysaccharide binding

Biological Process: immune system process; response to molecule of bacterial origin; innate immune response; positive regulation of endocytosis; inflammatory response; positive regulation of tumor necrosis factor production; positive regulation of cytokine secretion