Typical Testing Data/Standard Curve (for reference only)
Mouse Cluster of differentiation 100 ELISA Kit | CD100 elisa kit
Mouse Cluster of differentiation 100 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This CD100 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CD100. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CD100 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD100. Standards or samples are then added to the microtiter plate wells and CD100 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD100 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD100 are added to each well to “sandwich” the CD100 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzym e(HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD100 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD100 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9q22.2
Cellular Component: extracellular space; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding
Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; axon guidance; regulation of dendrite morphogenesis; negative regulation of cell adhesion; negative regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; immune response; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; positive regulation of cell migration; regulation of cell projection organization and biogenesis; negative regulation of apoptosis
Research Articles on CD100
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Product Notes
The Mouse CD100 sema4d (Catalog #AAA725813) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA725813 ELISA Kit recognizes Mouse CD100. It is sometimes possible for the material contained within the vial of "Cluster of differentiation 100, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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