Typical Testing Data/Standard Curve (for reference only)
Canine Colon Cancer specific antigene-3 ELISA Kit | CCSA-3 elisa kit
Canine Colon Cancer specific antigene-3 ELISA Kit
Gene Names
SDCCAG3; NY-CO-3
Reactivity
Canine
Synonyms
Colon Cancer specific antigene-3; Canine Colon Cancer specific antigene-3 ELISA Kit; CCSA-3 elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This CCSA-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CCSA-3. This ELISA kit for research use only, not for therapeutic applications
!Intended Uses
This CCSA-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine CCSA-3. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCSA-3 elisa kit
Principle of the Assay: CCSA-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCSA-3 antibody and an CCSA-3-HRP conjugate. The assay sample and buffer are incubated together with CCSA-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCSA-3 concentration since CCSA-3 from samples and CCSA-3-HRP conjugate compete for the anti-CCSA-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCSA-3 from the sample, fewer sites are left to bind CCSA-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCSA-3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,058 Da
NCBI Official Full Name
serologically defined colon cancer antigen 3 isoform 3
NCBI Official Synonym Full Names
serologically defined colon cancer antigen 3
NCBI Official Symbol
SDCCAG3
NCBI Official Synonym Symbols
NY-CO-3
NCBI Protein Information
serologically defined colon cancer antigen 3; antigen NY-CO-3
UniProt Protein Name
Serologically defined colon cancer antigen 3
UniProt Gene Name
SDCCAG3
UniProt Entry Name
SDCG3_HUMAN
Uniprot Description
SDCCAG3: May be involved in modulation of TNF response. May be involved in presentation of TNFRSF1A on the cell surface. Belongs to the SDCCAG3 family. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: recycling endosome; retromer complex; early endosome; midbody; endosome
Molecular Function: protein binding
Biological Process: protein transport; cell division; cell cycle; regulation of cytokinesis
Research Articles on CCSA-3
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Product Notes
The Canine CCSA-3 sdccag3 (Catalog #AAA752847) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA752847 ELISA Kit recognizes Canine CCSA-3. It is sometimes possible for the material contained within the vial of "Colon Cancer specific antigene-3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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