Cyclin D2 (CCND2) Recombinant Protein | CCND2 recombinant protein

Recombinant Cyclin D2 (CCND2)

Cat. Num. AAA2018503

• Gene Names: Ccnd2; cD2; Vin1; Vin-1; C86853; AI256817; BF642806; 2600016F06Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cyclin D2 (CCND2); Recombinant Cyclin D2 (CCND2); CCND2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QKDI QPYMRRMVAT WMLEVCEEQK CEEEVFPLAM NYLDRFLAGV PTPKTHLQLL GAVCMFLASK LKETIPLTAE KLCIYTDNSV KPQELLEWEL VVLGKLKWNL AAVTPHDFIE HILRKLPQQK EKLSLIRKHA QTFIALCATD FKFAMYPPSM IATGSVGAAI CGLQQDDEVN TLTCDALTEL LAKITHTDVDCLKACQEQIE ALLLNSLQQF RQEQHNA
• Sequence Length: 289

• Applicable Applications for CCND2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln47~Ala267 (Accession # P30280) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Nucleus. Cytoplasm. Membrane
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753310
• NCBI GeneID: 12444
• NCBI Accession #: NP_033959.1
• NCBI GenBank Nucleotide #: NM_009829.3
• UniProt Accession #: P30280
• Molecular Weight: 28.9kDa
• NCBI Official Full Name: G1/S-specific cyclin-D2
• NCBI Official Synonym Full Names: cyclin D2
• NCBI Official Symbol: Ccnd2
• NCBI Official Synonym Symbols: cD2; Vin1; Vin-1; C86853; AI256817; BF642806; 2600016F06Rik
• NCBI Protein Information: G1/S-specific cyclin-D2
• UniProt Protein Name: G1/S-specific cyclin-D2
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: Ccnd2
• UniProt Synonym Gene Names: Cyl-2
• UniProt Entry Name: CCND2_MOUSE

Uniprot Description

CCND2: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Belongs to the cyclin family. Cyclin D subfamily.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation

Cellular Component: nucleoplasm; nuclear membrane; membrane; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleolus; nucleus; chromatin; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: positive regulation of cyclin-dependent protein kinase activity; cell division; regulation of cell cycle; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; regulation of cyclin-dependent protein kinase activity; cell cycle; positive regulation of epithelial cell proliferation; G1/S transition of mitotic cell cycle