Typical Testing Data/Standard Curve (for reference only)
Porcine Cyclin D1 ELISA Kit | CCND1 elisa kit
Porcine Cyclin D1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CCND1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCND1 antibody and an CCND1-HRP conjugate. The assay sample and buffer are incubated together with CCND1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCND1 concentration since CCND1 from samples and CCND1-HRP conjugate compete for the anti-CCND1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCND1 from the sample, fewer sites are left to bind CCND1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCND1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.
Protein type: Nuclear receptor co-regulator; Cell cycle regulation; Oncoprotein; Activator
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; tight junction; transcriptional repressor complex; membrane; cyclin-dependent protein kinase holoenzyme complex; intracellular; cytosol; nucleus
Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; transcription factor binding; transcription corepressor activity; protein kinase binding; protein kinase activity
Biological Process: lactation; G1 DNA damage checkpoint; fat cell differentiation; establishment and/or maintenance of chromatin architecture; positive regulation of cyclin-dependent protein kinase activity; negative regulation of Wnt receptor signaling pathway; re-entry into mitotic cell cycle; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to vitamin E; response to magnesium ion; Leydig cell differentiation; response to iron ion; response to corticosterone stimulus; response to X-ray; response to drug; organ regeneration; Notch signaling pathway; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; liver development; response to organic nitrogen; response to ethanol; response to estrogen stimulus; cell division; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle
Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple
Research Articles on CCND1
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Product Notes
The Porcine CCND1 ccnd1 (Catalog #AAA743258) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA743258 ELISA Kit recognizes Porcine CCND1. It is sometimes possible for the material contained within the vial of "Cyclin D1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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