Cyclin B2 Recombinant Protein | CCNB2 recombinant protein

Recombinant Cyclin B2 (CCNB2)

Cat. Num. AAA2030773

• Gene Names: CCNB2; HsT17299
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cyclin B2; Recombinant Cyclin B2 (CCNB2); CCNB2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; VPV QPTKTTNVNK QLKPTASVKP VQMEKLAPKG PSPTPEDVSMKEENLCQAFS DALLCKIEDI DNEDWENPQL CSDYVKDIYQ YLRQLEVLQSINPHFLDGRD INGRMRAILV DWLVQVHSKF RLLQETLYMC VGIMDRFLQVQPVSRKKLQL VGITALLLAS KYEEMFSPNI EDFVYITDNA YTSSQIREMETLILKELKFE LGRPLPLHFL RRASKAGEVD VEQHTLAKYL MELTLIDYDMVHYHPSKVAA AASCLSQKVL GQGKWNLKQQ YYTGYTENEV LEVMQHMAKNVVKVNENLTK FIAIKNKYAS SKLLKISMIP QLNSKAVKDL ASPLIGRS
• Sequence Length: 374

• Applicable Applications for CCNB2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 42.7KDa
• Accurate Molecular Mass (KD): 43kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Val58~Ser398
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 371905558
• NCBI GeneID: 9133
• Molecular Weight: 45,282 Da
• NCBI Official Full Name: cyclin B2
• NCBI Official Synonym Full Names: cyclin B2
• NCBI Official Symbol: CCNB2
• NCBI Official Synonym Symbols: HsT17299
• NCBI Protein Information: G2/mitotic-specific cyclin-B2
• UniProt Protein Name: G2/mitotic-specific cyclin-B2
• Protein Family: G2/mitotic-specific cyclin
• UniProt Gene Name: CCNB2
• UniProt Entry Name: CCNB2_HUMAN

NCBI Description

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: nucleoplasm; microtubule cytoskeleton; centrosome; membrane; cytosol

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth