Myeloid Progenitor Inhibitory Factor 1 (MPIF1) Recombinant Protein | MPIF1 recombinant protein

Recombinant Myeloid Progenitor Inhibitory Factor 1 (MPIF1)

Cat. Num. AAA2012477

• Gene Names: CCL23; CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Myeloid Progenitor Inhibitory Factor 1 (MPIF1); Recombinant Myeloid Progenitor Inhibitory Factor 1 (MPIF1); MPIF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCVRMLKL DTRIKTRKN
• Sequence Length: 137

• Applicable Applications for MPIF1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Arg22~Asn120 (Accession # P55773) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 22538806
• NCBI GeneID: 6368
• NCBI Accession #: NP_005055.2
• NCBI GenBank Nucleotide #: NM_005064.4
• UniProt Accession #: P55773; O00174; O75950; Q52LD4; B7ZKQ3
• Molecular Weight: 12.9kDa
• NCBI Official Full Name: C-C motif chemokine 23 isoform CKbeta8-1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 23
• NCBI Official Symbol: CCL23
• NCBI Official Synonym Symbols: CKb8; MIP3; Ckb-8; MIP-3; MPIF-1; SCYA23; Ckb-8-1; hmrp-2a; CK-BETA-8
• NCBI Protein Information: C-C motif chemokine 23; C6 beta-chemokine; macrophage inflammatory protein 3; myeloid progenitor inhibitory factor 1; small inducible cytokine subfamily A (Cys-Cys), member 23
• UniProt Protein Name: C-C motif chemokine 23
• UniProt Gene Name: CCL23
• UniProt Synonym Gene Names: MIP3; MPIF1; SCYA23; CKB-8; MIP-3; MPIF-1
• UniProt Entry Name: CCL23_HUMAN

NCBI Description

This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; G-protein coupled receptor protein signaling pathway; negative regulation of cell proliferation; cell-cell signaling; immune response; signal transduction; chemotaxis; inflammatory response