MCP-1 recombinant protein

RECOMBINANT MOUSE MCP-1

Cat. Num. AAA232337

• Gene Names: Ccl2; JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE and HPLC analysis
• Synonyms: MCP-1; RECOMBINANT MOUSE MCP-1; MCP-1 recombinant protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Approximate Protein Concentration: 0.1mg/ml after reconstitution (varies by lot)
• Sequence Length: 148

• Applicable Applications for MCP-1 recombinant protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;; Functional Assays: Minimum Dilution: 1.0; Maximum Dilution: 20ng/ml;; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. ; Preparation
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for MCP-1 recombinant protein

Recombinant mouse MCP-1 is 13.8 kDa peptide corresponding to full-length mature mouse monocyte chemotactic protein 1. MCP-1, otherwise known as CCL2 or JE, is a secreted chemokine involved in the recruitment of monocytes to sites of injury or inflammation (Lu, B. et al., 1998).

NCBI and Uniprot Product Information

• NCBI GI #: 6755430
• NCBI GeneID: 20296
• NCBI Accession #: NP_035463.1
• NCBI GenBank Nucleotide #: NM_011333.3
• UniProt Accession #: P10148; Q9QYD7
• Molecular Weight: Protein Molecular Weight: 13.8kD (125 amino acid sequence)
• NCBI Official Full Name: C-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 2
• NCBI Official Symbol: Ccl2
• NCBI Official Synonym Symbols: JE; HC11; MCAF; MCP1; MCP-1; Scya2; Sigje; SMC-CF; AI323594
• NCBI Protein Information: C-C motif chemokine 2; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic protein 1; platelet-derived growth factor-inducible protein JE; small inducible cytokine A2; small-inducible cytokine A2
• UniProt Protein Name: C-C motif chemokine 2
• Protein Family: Major capsid protein
• UniProt Gene Name: Ccl2
• UniProt Synonym Gene Names: Je; Mcp1; Scya2; MCP-1
• UniProt Entry Name: CCL2_MOUSE

NCBI Description

This gene is one of several cytokine genes clustered on chromosome 11. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and memory T cells but not for neutrophils. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis. [provided by RefSeq, Sep 2015]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Chemokine; Secreted, signal peptide

Cellular Component: extracellular space; cell soma; rough endoplasmic reticulum; endocytic vesicle; perinuclear region of cytoplasm; cytoplasm; dendrite; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; chemokine activity; CCR2 chemokine receptor binding; cytokine activity

Biological Process: positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of collagen biosynthetic process; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; regulation of cell shape; transforming growth factor beta receptor signaling pathway; response to wounding; positive regulation of cell-cell adhesion; angiogenesis; inflammatory response; lymphocyte chemotaxis; neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; cytoskeleton organization and biogenesis; positive regulation of tumor necrosis factor production; glial cell migration; macrophage chemotaxis; leukocyte migration during inflammatory response; cellular calcium ion homeostasis; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; response to heat; eosinophil chemotaxis; positive regulation of endothelial cell proliferation; immune response; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase biosynthetic process