Bovine Cholecystokinin B Receptor ELISA Kit | CCKBR elisa kit

Bovine Cholecystokinin B Receptor ELISA Kit

Cat. Num. AAA752743

• Gene Names: CCKBR; GASR; CCK-B; CCK2R
• Reactivity: Bovine
• Synonyms: Cholecystokinin B Receptor; Bovine Cholecystokinin B Receptor ELISA Kit; CCKBR elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This CCKBR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CCKBR. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This CCKBR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CCKBR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCKBR elisa kit

Principle of the Assay: CCKBR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCKBR antibody and an CCKBR-HRP conjugate. The assay sample and buffer are incubated together with CCKBR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCKBR concentration since CCKBR from samples and CCKBR-HRP conjugate compete for the anti-CCKBR antibody binding site. Since the number of sites is limited, as more sites are occupied by CCKBR from the sample, fewer sites are left to bind CCKBR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCKBR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CCKBR elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 28316421
• NCBI GeneID: 887
• Molecular Weight: 41,504 Da
• NCBI Official Full Name: cholecystokinin-B receptor/gastrin receptor
• NCBI Official Synonym Full Names: cholecystokinin B receptor
• NCBI Official Symbol: CCKBR
• NCBI Official Synonym Symbols: GASR; CCK-B; CCK2R
• NCBI Protein Information: gastrin/cholecystokinin type B receptor; CCK-BR; CCK2-R; CCK2 receptor; CCK-B receptor; gastrin receptor; cholecystokinin-2 receptor
• UniProt Protein Name: Gastrin/cholecystokinin type B receptor
• Protein Family: Gastrin/cholecystokinin type B receptor
• UniProt Gene Name: CCKBR
• UniProt Synonym Gene Names: CCKRB; CCK-B receptor; CCK-BR; CCK2-R
• UniProt Entry Name: GASR_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

CCKBR: Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: type B gastrin/cholecystokinin receptor binding; protein binding; gastrin receptor activity; 1-phosphatidylinositol-3-kinase regulator activity; cholecystokinin receptor activity; phosphoinositide phospholipase C activity

Biological Process: gland development; gastric acid secretion; metabolic process; regulation of phosphoinositide 3-kinase activity; cell proliferation; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; gut development; sensory perception; digestion; positive regulation of cell proliferation; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating)