Human Cholecystokinin ELISA Kit | CCK elisa kit

Human Cholecystokinin ELISA Kit

Cat. Num. AAA765132

• Reactivity: Human
• Synonyms: Cholecystokinin; Human Cholecystokinin ELISA Kit; CCK (Cholecystokinin); CCK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CCK. No significant cross-reactivity or interference between CCK and analogues was observed.
• Sequence Length: 115

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for CCK elisa kit

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 292494892
• NCBI GeneID: 885
• NCBI Accession #: NP_001167609.1
• NCBI GenBank Nucleotide #: NM_001174138.2
• Molecular Weight: 12,669 Da
• NCBI Official Full Name: cholecystokinin preproprotein
• NCBI Official Synonym Full Names: cholecystokinin
• NCBI Official Symbol: CCK
• NCBI Protein Information: cholecystokinin
• UniProt Protein Name: Cholecystokinin
• Protein Family: Cholecystokinin
• UniProt Gene Name: CCK
• UniProt Synonym Gene Names: CCK; CCK58; CCK39; CCK33; CCK25; CCK18; CCK12; CCK8; CCK7; CCK5
• UniProt Entry Name: CCKN_HUMAN

NCBI Description

This gene encodes a member of the gastrin/cholecystokinin family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the peptide hormones cholecystokinin-8, -12, -33, and others. The encoded peptides have been shown to regulate gastric acid secretion and food intake. A sulfated form of cholecystokinin-8 may modulate neuronal activity in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]

Uniprot Description

CCK: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. Belongs to the gastrin/cholecystokinin family.

Protein type: Secreted; Hormone; Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: axon; axon hillock; dendrite; extracellular region; extracellular space; perikaryon; terminal button

Molecular Function: hormone activity; neuropeptide hormone activity; protein binding

Biological Process: axonogenesis; behavioral fear response; caspase activation; eating behavior; negative regulation of appetite; neuron migration; positive regulation of cell proliferation; positive regulation of glutamate secretion; positive regulation of mitochondrial depolarization; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein oligomerization; protein kinase C activation; regulation of sensory perception of pain; release of cytochrome c from mitochondria; signal transduction