Human Caspase 10 ELISA Kit | CASP10 elisa kit

Human Caspase 10 ELISA Kit

Cat. Num. AAA765638

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Reactivity: Human
• Synonyms: Caspase 10; Human Caspase 10 ELISA Kit; CASP10/FLICE2/Mch4/Apoptotic protease Mch-4/CASP-10/caspase 10; apoptosis-related cysteine peptidase/caspase 10; apoptosis-related cysteine protease/caspase-10/FADD-like ICE2/FAS-associated death domain protein interleukin-1B-converting enzyme 2/FLICE2/ICE-like apoptotic protease 4/interleukin-1B-converting enzyme 2/MCH4ALPS2; CASP10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CASP10. No significant cross-reactivity or interference between CASP10 and analogues was observed.
• Sequence Length: 478

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CASP10 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CASP10 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for CASP10 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CASP10 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CASP10 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CASP10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CASP10 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47419841
• NCBI GeneID: 843
• UniProt Accession #: Q92851; Q68HC0; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7
• Molecular Weight: 28,364 Da
• NCBI Official Full Name: caspase 10
• NCBI Official Synonym Full Names: caspase 10
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2
• UniProt Entry Name: CASPA_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.63; Protease

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: CD95 death-inducing signaling complex; cytosol

Molecular Function: cysteine-type endopeptidase activity; protein binding; ubiquitin protein ligase binding

Biological Process: apoptosis; cell surface receptor linked signal transduction; induction of apoptosis via death domain receptors; innate immune response; macrophage differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; proteolysis; regulation of apoptosis

Disease: Autoimmune Lymphoproliferative Syndrome, Type Iia; Gastric Cancer; Lymphoma, Non-hodgkin, Familial