Cocaine And Amphetamine Regulated Transcript Recombinant Protein | CART recombinant protein

Recombinant Cocaine And Amphetamine Regulated Transcript (CART)

Cat. Num. AAA2028886

• Gene Names: CARTPT; CART
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cocaine And Amphetamine Regulated Transcript; Recombinant Cocaine And Amphetamine Regulated Transcript (CART); CART recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; QED AELQPRALDI YSAVDDASHE KELPRRQLRA PGAVLQIEAL QEVLKKLKSK RIPIYEKKYGQVPMCDAGEQ CAVRKGARIG KLCDCPRGTS CNSFLLKCL
• Sequence Length: 116

• Applicable Applications for CART recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 41.4kDa
• Accurate Molecular Mass (KD): 41kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Gln28~Leu129
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4757910
• NCBI GeneID: 9607
• NCBI Accession #: NP_004282.1
• NCBI GenBank Nucleotide #: NM_004291.3
• Molecular Weight: 12,829 Da
• NCBI Official Full Name: cocaine- and amphetamine-regulated transcript protein preproprotein
• NCBI Official Synonym Full Names: CART prepropeptide
• NCBI Official Symbol: CARTPT
• NCBI Official Synonym Symbols: CART
• NCBI Protein Information: cocaine- and amphetamine-regulated transcript protein
• UniProt Protein Name: Cocaine- and amphetamine-regulated transcript protein
• Protein Family: Carcinine transporter
• UniProt Gene Name: CARTPT
• UniProt Synonym Gene Names: CART
• UniProt Entry Name: CART_HUMAN

NCBI Description

This gene encodes a secreted protein which is processed by prohormone/proprotein convertases to produce smaller, biologically active peptides. Expression of the transcript for this gene is regulated by certain drugs such as cocaine, and the encoded protein is thought to be involved in the regulation of appetite and stress. Mutations in this gene are associated with susceptibility to obesity.[provided by RefSeq, Nov 2009]

Uniprot Description

CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: extracellular space; secretory granule

Molecular Function: protein binding

Biological Process: negative regulation of osteoclast differentiation; activation of MAPKK activity; adult feeding behavior; signal transduction; cellular response to starvation; negative regulation of appetite; G-protein coupled receptor protein signaling pathway; synaptic transmission; cell-cell signaling; neuropeptide signaling pathway; positive regulation of epinephrine secretion; cell glucose homeostasis; circadian regulation of gene expression; negative regulation of bone resorption; regulation of insulin secretion; positive regulation of blood pressure; positive regulation of transmission of nerve impulse

Disease: Obesity