Calpain 3 Recombinant Protein | CAPN3 recombinant protein

Recombinant Calpain 3 (CAPN3)

Cat. Num. AAA2029372

• Gene Names: CAPN3; p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Calpain 3; Recombinant Calpain 3 (CAPN3); CAPN3 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; IIFVSDRAN SNKELGVDQE SEEGKGKTSP DKQKQSPQPQ PGSSDQESEE QQQFRNIFKQ IAGDDMEICA DELKKVLNTV VNKHKDLKTH GFTLESCRSM IALMDTDGSG KLNLQEFHHL WNKIKAWQKI FKHYDTDQSG TINSYEMRNA VNDAGFHLNN QLYDIITMRY ADKHMNIDFDSFICCFVRLE GMFRAFHAFD KDGDGIIKLN VLEWLQLTMY A
• Sequence Length: 729

• Applicable Applications for CAPN3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 29.1kDa
• Accurate Molecular Mass (KD): 32kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ile602~Ala821
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 7684607
• NCBI GeneID: 825
• Molecular Weight: 18,246 Da
• NCBI Official Full Name: calpain 3
• NCBI Official Synonym Full Names: calpain 3
• NCBI Official Symbol: CAPN3
• NCBI Official Synonym Symbols: p94; CANP3; LGMD2; nCL-1; CANPL3; LGMD2A
• NCBI Protein Information: calpain-3
• UniProt Protein Name: Calpain-3
• Protein Family: Calpain
• UniProt Gene Name: CAPN3
• UniProt Synonym Gene Names: CANP3; CANPL3; NCL1; CANP 3; nCL-1
• UniProt Entry Name: CAN3_HUMAN

NCBI Description

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq, Jul 2008]

Uniprot Description

CAPN3: Calcium-regulated non-lysosomal thiol-protease. Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A). LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset. Belongs to the peptidase C2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Calcium-binding; Protease; EC 3.4.22.54; Cell development/differentiation

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: protein complex; myofibril; cytoplasm; T-tubule; plasma membrane; intracellular; Z disc; cytosol; nucleus

Molecular Function: peptidase activity; protein binding; signal transducer activity; sodium ion binding; structural constituent of muscle; titin binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; protein complex scaffold; catalytic activity; ligase regulator activity; cysteine-type peptidase activity

Biological Process: response to muscle activity; muscle development; apoptosis; positive regulation of proteolysis; positive regulation of transcription, DNA-dependent; positive regulation of satellite cell activation involved in skeletal muscle regeneration; sarcomere organization; signal transduction; proteolysis; negative regulation of protein sumoylation; activation of NF-kappaB transcription factor; muscle maintenance; autolysis; myofibril assembly; regulation of catalytic activity; regulation of myoblast differentiation; protein complex assembly; positive regulation of release of sequestered calcium ion into cytosol; response to calcium ion; negative regulation of transcription, DNA-dependent; regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of apoptosis

Disease: Muscular Dystrophy, Limb-girdle, Type 2a