SDS-Page
Adenylyl Cyclase Associated Protein 2 Recombinant Protein | CAP2 recombinant protein
Recombinant Adenylyl Cyclase Associated Protein 2 (CAP2)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>90%
Synonyms
Adenylyl Cyclase Associated Protein 2; Recombinant Adenylyl Cyclase Associated Protein 2 (CAP2); CAP2 recombinant protein
Host
Host: E Coli
Source: Prokaryotic expression
Source: Prokaryotic expression
Purity/Purification
>90%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MANMQGLVER LERAVSRLES LSAESHRPPG NCGEVNGVIA GVAPSVEAFD KLMDSMVAEF LKNSRILAGD VETHAEMVHS AFQAQRAFLL MASQYQQPHE NDVAALLKPI SEKIQEIQTF RERNRGSNMF NHLSAVSESI PALGWIAVSP KPGPYVKEMN DAATFYTNRV LKDYKHSDLR HVDWVKSYLN IWSELQAYIK EHHTTGLTWS KTGPVASTVS AFSVLSSGPG LPPPPPPLPP PGPPPLFENE GKKEESSPSR SALFAQLNQG EAITKGLRHV TDDQKTYKNP SLRAQGGQTQ SPTKSHTPSP TSPKSYPSQK HAPVLELEGK KWRVEYQEDR NDLVISETEL KQVAYIFKCE KSTIQIKGKV NSIIIDNCKK LGLVFDNVVG IVEVINSQDI QIQVMGRVPT ISINKTEGCH IYLSEDALDC EIVSAKSSEM NILIPQDGDY REFPIPEQFK TAWDGSKLIT EPAEIMA
MANMQGLVER LERAVSRLES LSAESHRPPG NCGEVNGVIA GVAPSVEAFD KLMDSMVAEF LKNSRILAGD VETHAEMVHS AFQAQRAFLL MASQYQQPHE NDVAALLKPI SEKIQEIQTF RERNRGSNMF NHLSAVSESI PALGWIAVSP KPGPYVKEMN DAATFYTNRV LKDYKHSDLR HVDWVKSYLN IWSELQAYIK EHHTTGLTWS KTGPVASTVS AFSVLSSGPG LPPPPPPLPP PGPPPLFENE GKKEESSPSR SALFAQLNQG EAITKGLRHV TDDQKTYKNP SLRAQGGQTQ SPTKSHTPSP TSPKSYPSQK HAPVLELEGK KWRVEYQEDR NDLVISETEL KQVAYIFKCE KSTIQIKGKV NSIIIDNCKK LGLVFDNVVG IVEVINSQDI QIQVMGRVPT ISINKTEGCH IYLSEDALDC EIVSAKSSEM NILIPQDGDY REFPIPEQFK TAWDGSKLIT EPAEIMA
Sequence Length
477
Applicable Applications for CAP2 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
59.5kDa
Accurate Molecular Mass (KD)
60kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and S-tag
Organism Species
Homo sapiens (Human)
Fragment
Met1~Ala477
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,018 Da
NCBI Official Full Name
adenylyl cyclase-associated protein 2
NCBI Official Synonym Full Names
CAP, adenylate cyclase-associated protein, 2 (yeast)
NCBI Official Symbol
CAP2
NCBI Protein Information
adenylyl cyclase-associated protein 2
UniProt Protein Name
Adenylyl cyclase-associated protein 2
Protein Family
UniProt Gene Name
CAP2
UniProt Synonym Gene Names
CAP 2
UniProt Entry Name
CAP2_HUMAN
NCBI Description
This gene was identified by its similarity to the gene for human adenylyl cyclase-associated protein. The function of the protein encoded by this gene is unknown. However, the protein appears to be able to interact with adenylyl cyclase-associated protein and actin. [provided by RefSeq, Jul 2008]
Uniprot Description
CAP2: a cyclase-associated protein. CAPs are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is expressed mainly in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Up-regulated in human cancers. May play a role in multistage hepatocarcinogenesis.
Protein type: Actin-binding
Chromosomal Location of Human Ortholog: 6p22.3
Cellular Component: plasma membrane
Molecular Function: actin binding
Biological Process: axon guidance; adenylate cyclase activation; establishment and/or maintenance of cell polarity; cytoskeleton organization and biogenesis; signal transduction
Research Articles on CAP2
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Product Notes
The CAP2 cap2 (Catalog #AAA2031190) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adenylyl Cyclase Associated Protein 2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CAP2 cap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.MANMQGLVE R LERAVSRLES LSAESHRPPG NCGEVNGVIA GVAPSVEAFD KLMDSMVAEF LKNSRILAGD VETHAEMVHS AFQAQRAFLL MASQYQQPHE NDVAALLKPI SEKIQEIQTF RERNRGSNMF NHLSAVSESI PALGWIAVSP KPGPYVKEMN DAATFYTNRV LKDYKHSDLR HVDWVKSYLN IWSELQAYIK EHHTTGLTWS KTGPVASTVS AFSVLSSGPG LPPPPPPLPP PGPPPLFENE GKKEESSPSR SALFAQLNQG EAITKGLRHV TDDQKTYKNP SLRAQGGQTQ SPTKSHTPSP TSPKSYPSQK HAPVLELEGK KWRVEYQEDR NDLVISETEL KQVAYIFKCE KSTIQIKGKV NSIIIDNCKK LGLVFDNVVG IVEVINSQDI QIQVMGRVPT ISINKTEGCH IYLSEDALDC EIVSAKSSEM NILIPQDGDY REFPIPEQFK TAWDGSKLIT EPAEIMA. It is sometimes possible for the material contained within the vial of "Adenylyl Cyclase Associated Protein 2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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