Rat Adenylyl cyclase-associated protein 2 ELISA Kit | CAP2 elisa kit

Rat Adenylyl cyclase-associated protein 2 (CAP2) ELISA Kit

Cat. Num. AAA285266

• Reactivity: Rat
• Synonyms: Adenylyl cyclase-associated protein 2; Rat Adenylyl cyclase-associated protein 2 (CAP2) ELISA Kit; CAP2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat CAP2. No significant cross-reactivity or interference between Rat CAP2 and analogues was observed.
• Sequence Length: 477

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CAP2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CAP2 in samples. An antibody specific for CAP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 16758742
• NCBI GeneID: 116653
• NCBI Accession #: NP_446326.1
• NCBI GenBank Nucleotide #: NM_053874.1
• UniProt Accession #: P52481
• Molecular Weight: 52,912 Da
• NCBI Official Full Name: adenylyl cyclase-associated protein 2
• NCBI Official Synonym Full Names: cyclase associated actin cytoskeleton regulatory protein 2
• NCBI Official Symbol: Cap2
• NCBI Protein Information: adenylyl cyclase-associated protein 2
• UniProt Protein Name: Adenylyl cyclase-associated protein 2
• Protein Family: Adenylyl cyclase-associated protein
• UniProt Gene Name: Cap2
• UniProt Synonym Gene Names: CAP 2

NCBI Description

homologous to yeast adenylyl cyclase (CYR)-associated CAP protein; mutations in yeast homolog cause nutrition and temperature sensitivity, abnormal cell morphology, slow growth and abnormal actin distribution [RGD, Feb 2006]

Uniprot Description

CAP2: a cyclase-associated protein. CAPs are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is expressed mainly in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Up-regulated in human cancers. May play a role in multistage hepatocarcinogenesis.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 17p14

Cellular Component: cortical actin cytoskeleton; plasma membrane; postsynaptic density

Molecular Function: actin binding; adenylate cyclase binding; identical protein binding

Biological Process: actin cytoskeleton organization; actin polymerization or depolymerization; cell morphogenesis; cytoskeleton organization; establishment and/or maintenance of cell polarity; regulation of adenylate cyclase activity; signal transduction