Human Adenylyl Cyclase Associated Protein 1 ELISA Kit | CAP1 elisa kit

Human Adenylyl Cyclase Associated Protein 1 ELISA Kit

Cat. Num. AAA27308

• Gene Names: Park7; Dj1; CAP1; DJ-1; SP22
• Reactivity: Human
• Synonyms: Adenylyl Cyclase Associated Protein 1; Human Adenylyl Cyclase Associated Protein 1 ELISA Kit; CAP1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human CAP1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml – 2000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated CAP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human CAP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CAP1 and CAP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for CAP1 elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated CAP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human CAP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add CAP1 and CAP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

References

The clinical significance of protein expression of pancreatic neuroendocrine tumors-1 DJ. Lixin YANG, Lina ZHANG, Tianbiao ZHANG (2015)

NCBI and Uniprot Product Information

• NCBI GI #: 3250916
• NCBI GeneID: 117287
• UniProt Accession #: O88767
• Molecular Weight: 19,974 Da
• NCBI Official Full Name: CAP1
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: Park7
• NCBI Official Synonym Symbols: Dj1; CAP1; DJ-1; SP22
• NCBI Protein Information: protein DJ-1; fertility protein SP22; contraception-associated protein 1; parkinson disease protein 7 homolog; Parkinson disease (autosomal recessive, early onset) 7
• UniProt Protein Name: Protein DJ-1
• Protein Family: AP-1-like transcription factor
• UniProt Gene Name: Park7
• UniProt Synonym Gene Names: Cap1; Protein CAP1
• UniProt Entry Name: PARK7_RAT

NCBI Description

displays a specific localization pattern to the anterior-ventral surface of the equatorial segment; may play a role in sperm-egg interactions and fertilization [RGD, Feb 2006]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Tumor suppressor; Transcription regulation; Nuclear receptor co-regulator; EC 3.4.-.-

Cellular Component: PML body; mitochondrion; endoplasmic reticulum; axon; cytoplasm; plasma membrane; mitochondrial respiratory chain complex I; cytosol; nucleus; chromatin; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; peptidase activity; mRNA binding; protein binding; enzyme binding; copper ion binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding

Biological Process: synaptic transmission, dopaminergic; negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; response to drug; mitochondrion organization and biogenesis; protein stabilization; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of protein sumoylation; positive regulation of peptidyl-serine phosphorylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; methylglyoxal catabolic process to D-lactate; membrane depolarization; fertilization; response to hydrogen peroxide; detoxification of mercury ion; regulation of inflammatory response; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; lactate biosynthetic process; negative regulation of protein kinase activity; autophagy; positive regulation of transcription factor activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of apoptosis