Canine Syndecan 1 (SDC1) ELISA Kit | SDC1 elisa kit

Canine Syndecan 1 (SDC1) ELISA Kit

Cat. Num. AAA2611112

• Gene Names: SDC1; SDC; CD138; SYND1; syndecan
• Reactivity: Canine
• Synonyms: Syndecan 1 (SDC1); Canine Syndecan 1 (SDC1) ELISA Kit; SDC1 elisa kit

• Reactivity: Canine
• Sequence Length: 310

• Samples: Canine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 ng/mL-1.56 ng/mL
• Sensitivity: Up to 0.5 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for SDC1 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine SDC1 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 1519316397
• NCBI GeneID: 6382
• NCBI Accession #: NP_002988.4
• NCBI GenBank Nucleotide #: NM_002997.5
• UniProt Accession #: P18827; Q53QV0; Q546D3; Q96HB7; D6W523
• Molecular Weight: 32,462 Da
• NCBI Official Full Name: syndecan-1
• NCBI Official Synonym Full Names: syndecan 1
• NCBI Official Symbol: SDC1
• NCBI Official Synonym Symbols: SDC; CD138; SYND1; syndecan
• NCBI Protein Information: syndecan-1
• UniProt Protein Name: Syndecan-1
• Protein Family: Syndecan
• UniProt Gene Name: SDC1
• UniProt Synonym Gene Names: SDC; SYND1

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413).