Canine Fas/APO-1 ELISA Kit | Fas/APO-1 elisa kit

Canine Fas/APO-1 ELISA Kit

Cat. Num. AAA738832

• Gene Names: FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Reactivity: Canine
• Synonyms: Fas/APO-1; Canine Fas/APO-1 ELISA Kit; Fas/APO-1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This FAS/APO-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine FAS/APO-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Fas/APO-1 elisa kit

Principle of the Assay: FAS/APO-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FAS/APO-1 antibody and an FAS/APO-1-HRP conjugate. The assay sample and buffer are incubated together with FAS/APO-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAS/APO-1 concentration since FAS/APO-1 from samples and FAS/APO-1-HRP conjugate compete for the anti-FAS/APO-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FAS/APO-1 from the sample, fewer sites are left to bind FAS/APO-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAS/APO-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Fas/APO-1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 887458
• NCBI GeneID: 355
• UniProt Accession #: P25445; Q14292; Q14293; Q14294; Q14295; Q16652; Q5T9P1; Q5T9P2; Q5T9P3; Q6SSE9; A9UJX4; B6VNV4
• Molecular Weight: 24,781 Da
• NCBI Official Full Name: Fas/Apo-1/CD95
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: FAS
• NCBI Official Synonym Symbols: APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; FAS receptor variant 9; APO-1 cell surface antigen; TNF receptor superfamily member 6; apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); tumor necrosis factor receptor superfamily, member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• UniProt Gene Name: FAS
• UniProt Synonym Gene Names: APT1; FAS1; TNFRSF6
• UniProt Entry Name: TNR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Uniprot Description

FAS: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD. Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Apoptosis; Receptor, cytokine

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: cell surface; cytoplasm; integral to membrane; plasma membrane; CD95 death-inducing signaling complex; cytosol; nucleus; external side of plasma membrane; lipid raft

Molecular Function: identical protein binding; protein binding; signal transducer activity; transmembrane receptor activity; receptor activity; kinase binding

Biological Process: spleen development; caspase activation; circadian rhythm; regulation of myeloid cell differentiation; renal system process; transformed cell apoptosis; positive regulation of apoptosis; apoptosis; positive regulation of protein homooligomerization; response to toxin; regulation of lymphocyte differentiation; response to glucocorticoid stimulus; negative regulation of B cell activation; signal transduction; negative thymic T cell selection; regulation of apoptosis; B cell mediated immunity; inflammatory cell apoptosis; induction of apoptosis via death domain receptors; protein complex assembly; gene expression; immunoglobulin production; activated T cell apoptosis; protein homooligomerization; negative regulation of apoptosis

Disease: Autoimmune Lymphoproliferative Syndrome