Canine Amylin ELISA Kit | IAPP elisa kit

Canine Amylin ELISA Kit

Cat. Num. AAA2605132

• Gene Names: IAPP; DAP; IAP
• Reactivity: Canine
• Synonyms: Amylin; Canine Amylin ELISA Kit; IAPP elisa kit

• Reactivity: Canine
• Sequence Length: 62

• Samples: Canine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 ng/mL-1.56 ng/mL
• Sensitivity: Up to 0.5 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IAPP elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine Amylin monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 457131
• NCBI GeneID: 3375
• Molecular Weight: 9,806 Da
• NCBI Official Full Name: amylin, partial
• NCBI Official Synonym Full Names: islet amyloid polypeptide
• NCBI Official Symbol: IAPP
• NCBI Official Synonym Symbols: DAP; IAP
• NCBI Protein Information: islet amyloid polypeptide
• UniProt Protein Name: Islet amyloid polypeptide
• Protein Family: Islet amyloid polypeptide
• UniProt Gene Name: IAPP
• UniProt Synonym Gene Names: DAP
• UniProt Entry Name: IAPP_HUMAN

NCBI Description

The protein encoded by this gene is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. Studies suggest that this protein, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. This protein also exhibits an bactericidal, antimicrobial activity. [provided by RefSeq, Sep 2014]

Uniprot Description

IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: cell soma; extracellular region; extracellular space

Molecular Function: hormone activity; identical protein binding; receptor binding

Biological Process: apoptosis; cell-cell signaling; cellular protein metabolic process; eating behavior; endocrine pancreas development; negative regulation of bone resorption; negative regulation of cell differentiation; sensory perception of pain; signal transduction