Typical Testing Data/Standard Curve (for reference only)
Canine Alpha-2-macroglobulin-P (A2MP) ELISA Kit | A2MP elisa kit
Canine Alpha-2-macroglobulin-P (A2MP) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: A1ACT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-A1ACT antibody and an A1ACT-HRP conjugate. The assay sample and buffer are incubated together with A1ACT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A1ACT concentration since A1ACT from samples and A1ACT-HRP conjugate compete for the anti-A1ACT antibody binding site. Since the number of sites is limited, as more sites are occupied by A1ACT from the sample, fewer sites are left to bind A1ACT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A1ACT concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.
Protein type: Secreted, signal peptide; Inhibitor; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; protease inhibitor activity; growth factor binding; endopeptidase inhibitor activity; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding
Biological Process: negative regulation of complement activation, lectin pathway; negative regulation of peptidase activity; stem cell differentiation; female pregnancy
Research Articles on A2MP
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Product Notes
The Canine A2MP a2mp (Catalog #AAA7211675) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211675 ELISA Kit recognizes Canine A2MP. It is sometimes possible for the material contained within the vial of "Alpha-2-macroglobulin-P (A2MP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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