Canine Osteopontin (OPN) DIY ELISA Kit | OPN diy elisa kit

Canine Osteopontin (OPN) ELISA Kit DIY Materials

Cat. Num. AAA2088919

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1
• Reactivity: Canine
• Synonyms: Osteopontin (OPN); Canine Osteopontin (OPN) ELISA Kit DIY Materials; SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin; OPN diy elisa kit

• Reactivity: Canine
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Osteopontin (OPN). No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.
• Sequence Length: 300

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for OPN diy elisa kit

Tumor immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 189405
• NCBI GeneID: 6696
• Molecular Weight: 33,843 Da
• NCBI Official Full Name: osteopontin
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1
• NCBI Protein Information: osteopontin
• UniProt Protein Name: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1

NCBI Description

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular region; extracellular space

Molecular Function: extracellular matrix binding; protein binding

Biological Process: cell adhesion; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; osteoblast differentiation; positive regulation of bone resorption; response to vitamin D