Calcium/Calmodulin Dependent Protein Kinase II Beta Recombinant Protein | CAMK2b recombinant protein

Recombinant Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b)

Cat. Num. AAA2029885

• Gene Names: CAMK2B; CAM2; CAMK2; CAMKB
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Calcium/Calmodulin Dependent Protein Kinase II Beta; Recombinant Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b); CAMK2b recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MATTVTCTRF TDEYQLYEDI GKGAFSVVRR CVKLCTGHEY AAKIINTKKLSARDHQKLER EARICRLLKH SNIVRLHDSI SEEGFHYLVF DLVTGGELFEDIVAREYYSE ADASHCIQQI LEAVLHCHQM GVVHRDLKPE NLLLASKCKGAAVKLADFGL AIEVQGDQQA WFGFAGTPGY LSPEVLRKEA YGKPVDIWACGVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT VTPEAKNLINQMLTINPAKR ITAHEALKHP WVCQRSTVAS MMHRQETVEC LKKFNARRKLK
• Sequence Length: 503

• Applicable Applications for CAMK2b recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 38.0kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Met1~Lys301
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 17512173
• NCBI GeneID: 816
• Molecular Weight: 57,938 Da
• NCBI Official Full Name: Calcium/calmodulin-dependent protein kinase II beta
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II beta
• NCBI Official Symbol: CAMK2B
• NCBI Official Synonym Symbols: CAM2; CAMK2; CAMKB
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit beta
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit beta
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2B
• UniProt Synonym Gene Names: CAM2; CAMK2; CAMKB; CaM kinase II subunit beta; CaMK-II subunit beta
• UniProt Entry Name: KCC2B_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CAMK2B: a widely expressed protein kinase of the calcium/calmodulin-dependent protein kinase (CAMK) group. Functions autonomously after Ca2+/calmodulin-binding and autophosphorylation. Involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban, an endogenous inhibitor of SERCA2. The holoenzyme is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2. Interacts with MPDZ. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle and induced during exercise. Eight isoforms of the human protein are produced by alternative splicing

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.17; Kinase, protein; Protein kinase, CAMK; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 7p14.3-p14.1

Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; plasma membrane; microtubule organizing center; spindle midzone; cytosol

Molecular Function: calmodulin binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; actin binding; ATP binding

Biological Process: regulation of long-term neuronal synaptic plasticity; regulation of skeletal muscle adaptation; cytokine and chemokine mediated signaling pathway; protein amino acid autophosphorylation; regulation of calcium ion transport; signal transduction; protein amino acid phosphorylation; regulation of synaptic transmission, cholinergic; inhibitory G-protein coupled receptor phosphorylation; synaptic transmission; peptidyl-serine phosphorylation; response to cadmium ion; calcium ion transport; regulation of synapse structural plasticity; neuromuscular process controlling balance; G1/S transition of mitotic cell cycle