CAMK1D recombinant protein

Recombinant Human CAMK1D Protein

Cat. Num. AAA8248659

• Gene Names: CAMK1D; CKLiK; CaM-K1; CaMKID
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CAMK1D; Recombinant Human CAMK1D Protein; CAMKID; Calcium/calmodulin-dependent protein kinase type 1D; CaM kinase I delta; CaM kinase ID; CaM-KI delta; CaMKI delta; CaMKID; CaMKI-like protein kinase; CKLiK; CAMK1D recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMARE NGESSSSWKK QAEDIKKIFE FKETLGTGAF SEVVLAEEKA TGKLFAVKCI PKKALKGKES SIENEIAVLR KIKHENIVAL EDIYESPNHL YLVMQLVSGG ELFDRIVEKG FYTEKDASTL IRQVLDAVYY LHRMGIVHRD LKPENLLYYS QDEESKIMIS DFGLSKMEGK GDVMSTACGT PGYVAPEVLA QKPYSKAVDC WSIGVIAYIL LCGYPPFYDE NDSKLFEQIL KAEYEFDSPY WDDISDSAKD FIRNLMEKDP NKRYTCEQAA RHPWIAGDTA LNKNIHESVS AQIRKNFAKS KWRQAFNATA VVRHMRKLHL GSSLDSSNAS VSSSLSLASQ KDCLAPSTLC SFISSSSGVS GVGAERRPRP TTVTAVHSGS K
• Sequence Length: 357

• Applicable Applications for CAMK1D recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CAMK1D recombinant protein

Recombinant Human CAMK1D Protein is produced by our E Coli expression system and the target gene encoding Met1-Lys385 is expressed with a GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 9966875
• NCBI GeneID: 57118
• NCBI Accession #: NP_065130.1
• NCBI GenBank Nucleotide #: NM_020397.3
• UniProt Accession #: Q8IU85; Q9HD31; B0YIY0
• Molecular Weight: 40,190 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type 1D isoform 1
• NCBI Official Synonym Full Names: calcium/calmodulin dependent protein kinase ID
• NCBI Official Symbol: CAMK1D
• NCBI Official Synonym Symbols: CKLiK; CaM-K1; CaMKID
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type 1D
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type 1D
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK1D
• UniProt Synonym Gene Names: CAMKID; CaM kinase ID; CaM-KI delta; CaMKI delta; CaMKID; CKLiK
• UniProt Entry Name: KCC1D_HUMAN

NCBI Description

This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of the serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It has been associated with multiple processes including regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophil cells, and apoptosis of erythroleukemia cells. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jan 2015]

Uniprot Description

CAMK1D: a calcium/calmodulin-dependent S/T protein kinase of the CAMK group. Binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CAMKK1 and CAMKK2. Can be activated by CAMKK1 in a calcium-independent manner. Its Ca(2+)/CaM-dependent activity is enhanced 30-fold in vitro by phosphorylation at Thr180 by CaMKK1. Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1. Expression increases upon treatment of EC cells with DMSO and retinoic acid. Two isoforms of the human protein are produced by alternative splicing.

Protein type: CAMK group; CAMK1 family; EC 2.7.11.17; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleus

Molecular Function: calmodulin binding; calmodulin-dependent protein kinase activity

Biological Process: activation of CREB transcription factor; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of phagocytosis; regulation of dendrite development