Calcyon Neuron Specific Vesicular Protein Recombinant Protein | CALY recombinant protein

Recombinant Calcyon Neuron Specific Vesicular Protein (CALY)

Cat. Num. AAA2034590

• Gene Names: CALY; NSG3; DRD1IP
• Reactivity: Rat
• Applications: ELISA, Immunoprecipitation, SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Calcyon Neuron Specific Vesicular Protein; Recombinant Calcyon Neuron Specific Vesicular Protein (CALY); Dopamine Receptor D1 Interacting Protein; CALY recombinant protein

• Host: E Coli; Source: Prokaryotic expression
• Reactivity: Rat
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 217

• Applicable Applications for CALY recombinant protein: SDS-PAGE; Western Blot (WB); ELISA (EIA); Immunoprecipitation (IP)
• Usage: Reconstitute in PBS or others.
• Endotoxin Level: < 1.0EU per 1ug (determined by the LAL method)
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Fragment: Asp110~Lys226
• Tag: Two N-terminal Tags, His-tag and T7-tag
• Residues & Tags: Asp110~Lys226 with N-terminal His Tag
• Subcellular Location: Secreted
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 24659432
• NCBI GeneID: 50632
• Molecular Weight: Predicted: 16.5kDa
• NCBI Official Full Name: Calcyon neuron-specific vesicular protein
• NCBI Official Synonym Full Names: calcyon neuron specific vesicular protein
• NCBI Official Symbol: CALY
• NCBI Official Synonym Symbols: NSG3; DRD1IP
• NCBI Protein Information: neuron-specific vesicular protein calcyon
• UniProt Protein Name: Neuron-specific vesicular protein calcyon
• Protein Family: Neuron-specific vesicular protein calcyon
• UniProt Gene Name: CALY
• UniProt Synonym Gene Names: DRD1IP
• UniProt Entry Name: CALY_HUMAN

NCBI Description

The protein encoded by this gene is a type II single transmembrane protein. It is required for maximal stimulated calcium release after stimulation of purinergic or muscarinic but not beta-adrenergic receptors. The encoded protein interacts with D1 dopamine receptor and may interact with other DA receptor subtypes and/or GPCRs. [provided by RefSeq, Jul 2008]

Uniprot Description

calcyon: Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis. Belongs to the NSG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: cytoplasmic vesicle; integral to plasma membrane

Molecular Function: clathrin light chain binding; protein binding

Biological Process: clathrin cage assembly; positive regulation of endocytosis