CALM2 recombinant protein

CALM2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121167

• Gene Names: CALM2; caM; PHKD; CAMII; LQT15; PHKD2
• Purity: >85% as determined by SDS-PAGE
• Synonyms: CALM2; CALM2 Protein; Human; Recombinant (His Tag); Human Calmodulin 2/CALM2 Protein (His Tag); caM Protein; CAMII Protein; LQT15 Protein; PHKD Protein; PHKD2 Protein; calmodulin 2 (phosphorylase kinase; delta); CALM2 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys149

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human CALM2 (P0DP24)(Met1-Lys149) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CALM2 recombinant protein

Background: Calmodulin 2, also known as CALM2, is a calmodulin. Calmodulin 2 mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). It is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin 2 gene may be a genetic determinant of hip osteoarthritis (OA). OA is a degenerative disease characterized by gradual loss of articular cartilage and is a leading cause of disability in elderly populations. CALM2 was most abundantly expressed in articular chondrocytes and OA cartilage.

Product Categories/Family for CALM2 recombinant protein

Actin Dynamics Signaling Pathway

References

Egli R, et al. (1993) Localization of the human bona fide calmodulin genes CALM1, CALM2, and CALM3 to chromosomes 14q24-q31, 2p21.1-p21.3, and 19q13.2-q13.3. Genomics. 16(2): 461-5.Mikiko, et al. (2002) Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex. Mol Biol Cell. 13(9):3235-45.SenGupta B, et al. (1987) Molecular analysis of human and rat calmodulin complementary DNA clones. Evidence for additional active genes in these species. J Biol Chem. 262(34): 16663-70.

NCBI and Uniprot Product Information

• NCBI GI #: 4502549
• NCBI GeneID: 805
• NCBI Accession #: NP_001734
• NCBI GenBank Nucleotide #: NM_001743.5
• UniProt Accession #: P0DP24; P02593; P62158; P70667; P99014; Q13942; Q53S29; Q61379; Q61380; Q96HK3
• Molecular Weight: 16,838 Da
• NCBI Official Full Name: calmodulin-2 isoform 2
• NCBI Official Synonym Full Names: calmodulin 2
• NCBI Official Symbol: CALM2
• NCBI Official Synonym Symbols: caM; PHKD; CAMII; LQT15; PHKD2
• NCBI Protein Information: calmodulin-2
• UniProt Protein Name: Calmodulin-2
• UniProt Gene Name: CALM2

NCBI Description

This gene is a member of the calmodulin gene family. There are three distinct calmodulin genes dispersed throughout the genome that encode the identical protein, but differ at the nucleotide level. Calmodulin is a calcium binding protein that plays a role in signaling pathways, cell cycle progression and proliferation. Several infants with severe forms of long-QT syndrome (LQTS) who displayed life-threatening ventricular arrhythmias together with delayed neurodevelopment and epilepsy were found to have mutations in either this gene or another member of the calmodulin gene family (PMID:23388215). Mutations in this gene have also been identified in patients with less severe forms of LQTS (PMID:24917665), while mutations in another calmodulin gene family member have been associated with catecholaminergic polymorphic ventricular tachycardia (CPVT)(PMID:23040497), a rare disorder thought to be the cause of a significant fraction of sudden cardiac deaths in young individuals. Pseudogenes of this gene are found on chromosomes 10, 13, and 17. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding. Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed:16760425). Mediates calcium-dependent inactivation of CACNA1C (PubMed:26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed:27165696).